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Literature DB >> 2538185

The failure of endothelin to displace bound, radioactively-labelled, calcium antagonists (PN 200/110, D888 and diltiazem).

X H Gu¹, J J Liu, J S Dillon, W G Nayler.

Abstract

The effect of endothelin, a potent vasoconstrictor polypeptide, on three types of calcium antagonist binding sites was examined, in rat cardiac membrane fragments. Endothelin 10 nM affected neither the affinity nor density of dihydropyridine binding sites. At concentrations of 10(-12)-10(-7) M, endothelin failed to displace bound (+)-[3H]-PN 200/110, (-)-[3H]-D888 and (+)-cis-[3H]-diltiazem. These results suggest that the calcium antagonist binding sites associated with L-type calcium channels are not the primary site of action of endothelin.

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Year: 1989 PMID： 2538185 PMCID： PMC1854350 DOI： 10.1111/j.1476-5381.1989.tb11811.x

Source DB: PubMed Journal: Br J Pharmacol ISSN： 0007-1188 Impact factor: 8.739

9 in total

1. Protein measurement with the Folin phenol reagent.

Authors: O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal: J Biol Chem Date: 1951-11 Impact factor: 5.157

2. Assay for calcium channels.

Authors: H Glossmann; D R Ferry
Journal: Methods Enzymol Date: 1985 Impact factor: 1.600

3. Effects of endothelin on the renal artery from spontaneously hypertensive and Wistar Kyoto rats.

Authors: Y Tomobe; T Miyauchi; A Saito; M Yanagisawa; S Kimura; K Goto; T Masaki
Journal: Eur J Pharmacol Date: 1988-08-02 Impact factor: 4.432

4. A novel potent vasoconstrictor peptide produced by vascular endothelial cells.

Authors: M Yanagisawa; H Kurihara; S Kimura; Y Tomobe; M Kobayashi; Y Mitsui; Y Yazaki; K Goto; T Masaki
Journal: Nature Date: 1988-03-31 Impact factor: 49.962

5. Dihydropyridine binding sites in aerobically perfused, ischemic, and reperfused rat hearts: effect of temperature and time.

Authors: X H Gu; J S Dillon; W G Nayler
Journal: J Cardiovasc Pharmacol Date: 1988-09 Impact factor: 3.105

6. Ligand: a versatile computerized approach for characterization of ligand-binding systems.

Authors: P J Munson; D Rodbard
Journal: Anal Biochem Date: 1980-09-01 Impact factor: 3.365

7. A practical computer-based approach to the analysis of radioligand binding experiments.

Authors: G A McPherson
Journal: Comput Programs Biomed Date: 1983 Aug-Oct

8. Endothelial cells in culture produce a vasoconstrictor substance.

Authors: R F O'Brien; R J Robbins; I F McMurtry
Journal: J Cell Physiol Date: 1987-08 Impact factor: 6.384

9. Sustained coronary vasoconstriction provoked by a peptidergic substance released from endothelial cells in culture.

Authors: M N Gillespie; J O Owasoyo; I F McMurtry; R F O'Brien
Journal: J Pharmacol Exp Ther Date: 1986-02 Impact factor: 4.030

9 in total

10 in total

The failure of endothelin to displace bound, radioactively-labelled, calcium antagonists (PN 200/110, D888 and diltiazem).

1. Protein measurement with the Folin phenol reagent.

2. Assay for calcium channels.

3. Effects of endothelin on the renal artery from spontaneously hypertensive and Wistar Kyoto rats.

4. A novel potent vasoconstrictor peptide produced by vascular endothelial cells.

5. Dihydropyridine binding sites in aerobically perfused, ischemic, and reperfused rat hearts: effect of temperature and time.

6. Ligand: a versatile computerized approach for characterization of ligand-binding systems.

7. A practical computer-based approach to the analysis of radioligand binding experiments.

8. Endothelial cells in culture produce a vasoconstrictor substance.

9. Sustained coronary vasoconstriction provoked by a peptidergic substance released from endothelial cells in culture.

1. Nifedipine and experimental cardioprotection.

Review 2. Endothelin: a review of its effects and possible mechanisms of action.

3. Inability of endothelin to increase Ca2+ current in guinea-pig heart cells.

4. Endothelin binding to cultured calf adrenal zona glomerulosa cells and stimulation of aldosterone secretion.

5. Mechanism of endothelin-induced contraction in guinea-pig trachea: comparison with rat aorta.

6. The pharmacological properties of the peptide, endothelin.

7. Endothelin-1 inhibits PAF-induced paw oedema and pleurisy in the mouse.

8. Potentiation by endothelin-1 of 5-hydroxytryptamine-induced contraction in coronary artery of the pig.

9. Interaction between endothelin and vasodilators in the human internal mammary artery.

10. Endothelin-1 inhibits pre-stimulated tracheal submucosal gland secretion and epithelial albumin transport.