J Yin1, F Zeng, N Wu, K Kang, Z Yang, H Yang. 1. Department of Gynecology, The Ninth People's Hospital of Chongqing, 69 JiaLing Road, Chongqing, 400700, People's Republic of China.
Abstract
PURPOSE: It has been well established that high serum levels of interleukin-8 (IL-8) in ovarian cancer result in a poor clinical outcome. Thus, the aim of this study was investigating the role of IL-8 in ovarian cancer development. METHODS: Two human ovarian cancer cell lines (SKOV3 and OVCAR3) were cocultured with IL-8 (100 ng/L) for 24 h, then cell migration was determined by transwell assay. Epithelial-mesenchymal transition (EMT)-associated proteins including E-cadherin and β-catenin, and phosphorylation status of β-catenin were investigated by Western blot analysis. RESULTS: After treatment with IL-8 (100 ng/L) for 24 h, transwell assay result showed that the number of migrated ovarian cells increased significantly. Western blot analysis revealed that protein levels of E-cadherin were decreased, while that of β-catenin were elevated both in IL-8 pretreated SKOV3 and OVCAR3 cells. We further found that phosphorylation status of β-catenin were elevated either in cytoplasm or in nucleus of these two ovarian cancer cell lines after treatment with IL-8 for 24 h. CONCLUSIONS: Our data suggest that IL-8 induces EMT in ovarian cancer cells and implicates its potential role in enhancing ovarian cancer cell metastasis.
PURPOSE: It has been well established that high serum levels of interleukin-8 (IL-8) in ovarian cancer result in a poor clinical outcome. Thus, the aim of this study was investigating the role of IL-8 in ovarian cancer development. METHODS: Two humanovarian cancer cell lines (SKOV3 and OVCAR3) were cocultured with IL-8 (100 ng/L) for 24 h, then cell migration was determined by transwell assay. Epithelial-mesenchymal transition (EMT)-associated proteins including E-cadherin and β-catenin, and phosphorylation status of β-catenin were investigated by Western blot analysis. RESULTS: After treatment with IL-8 (100 ng/L) for 24 h, transwell assay result showed that the number of migrated ovarian cells increased significantly. Western blot analysis revealed that protein levels of E-cadherin were decreased, while that of β-catenin were elevated both in IL-8 pretreated SKOV3 and OVCAR3 cells. We further found that phosphorylation status of β-catenin were elevated either in cytoplasm or in nucleus of these two ovarian cancer cell lines after treatment with IL-8 for 24 h. CONCLUSIONS: Our data suggest that IL-8 induces EMT in ovarian cancer cells and implicates its potential role in enhancing ovarian cancer cell metastasis.
Authors: Sung-Woo Kim; Masaaki Hayashi; Jeng-Fan Lo; Colleen Fearns; Rong Xiang; Gwendal Lazennec; Young Yang; Jiing-Dwan Lee Journal: Cancer Res Date: 2005-10-01 Impact factor: 12.701
Authors: Andreas Eger; Andreas Stockinger; John Park; Elke Langkopf; Mario Mikula; Josef Gotzmann; Wolfgang Mikulits; Hartmut Beug; Roland Foisner Journal: Oncogene Date: 2004-04-08 Impact factor: 9.867
Authors: N J Sullivan; A K Sasser; A E Axel; F Vesuna; V Raman; N Ramirez; T M Oberyszyn; B M Hall Journal: Oncogene Date: 2009-07-06 Impact factor: 9.867