Balakrishnan Aristatile1, Abdullah H Al-Assaf1, Kodukkur Viswanathan Pugalendi2. 1. Department of Food Science and Nutrition, College of Food and Agricultural Science, King Saud University, P.O. Box 2460, Riyadh 11451, Saudi Arabia. 2. Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalainagar - 608 002, Tamilnadu, India.
Abstract
BACKGROUND: Carvacrol (2-methyl-5-(1-methylethyl)-phenol) is a predominant monoterpenic phenol which occurs in many essential oils of the family Labiatae including Origanum, Satureja, Thymbra, Thymus, and Corydothymus species. It is well known for its anti-inflammatory, antioxidant and antitumor activities. The present study investigates the influence of carvacrol on CYP2E1 and PPAR-α on D-Galactosamine (D-GalN)-induced hepatotoxic rats. MATERIALS AND METHODS: The mRNA and protein expression levels of CYP2E1 and PPAR-α have been assayed by semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) and western blot analysis. RESULT: The result demonstrated that the mRNA and protein expressions of CYP2E1(p=0.012; p=0.015) significantly up-regulated while the mRNA and protein expressions of PPAR-α (p=0.026; p=0.03) significantly down-regulated on D-galactosamine induced hepatotoxic rats and treatment with carvacrol significantly suppressed the mRNA and protein (CYP2E1, p=0.010; p=0.011) (PPAR-α, p=0.033; p=0.037) expressions of these genes. CONCLUSION: Thus, the present results have shown that carvacrol has the hepatoprotective effect and also alleviates liver damage associated with GalN induced hepatotoxic rats by down-regulating the CYP2E1 and up-regulating the PPAR-α expression.
BACKGROUND:Carvacrol (2-methyl-5-(1-methylethyl)-phenol) is a predominant monoterpenicphenol which occurs in many essential oils of the family Labiatae including Origanum, Satureja, Thymbra, Thymus, and Corydothymus species. It is well known for its anti-inflammatory, antioxidant and antitumor activities. The present study investigates the influence of carvacrol on CYP2E1 and PPAR-α on D-Galactosamine (D-GalN)-induced hepatotoxicrats. MATERIALS AND METHODS: The mRNA and protein expression levels of CYP2E1 and PPAR-α have been assayed by semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) and western blot analysis. RESULT: The result demonstrated that the mRNA and protein expressions of CYP2E1(p=0.012; p=0.015) significantly up-regulated while the mRNA and protein expressions of PPAR-α (p=0.026; p=0.03) significantly down-regulated on D-galactosamine induced hepatotoxicrats and treatment with carvacrol significantly suppressed the mRNA and protein (CYP2E1, p=0.010; p=0.011) (PPAR-α, p=0.033; p=0.037) expressions of these genes. CONCLUSION: Thus, the present results have shown that carvacrol has the hepatoprotective effect and also alleviates liver damage associated with GalN induced hepatotoxicrats by down-regulating the CYP2E1 and up-regulating the PPAR-α expression.
Authors: Ji-Ming Ye; Miguel A Iglesias; David G Watson; Bronwyn Ellis; Leonie Wood; Per Bo Jensen; Rikke Veggerby Sørensen; Philip Just Larsen; Gregory J Cooney; Karsten Wassermann; Edward W Kraegen Journal: Am J Physiol Endocrinol Metab Date: 2003-03 Impact factor: 4.310
Authors: Marie Stiborová; Lucie Borek-Dohalská; Petr Hodek; Jaroslav Mráz; Eva Frei Journal: Arch Biochem Biophys Date: 2002-07-01 Impact factor: 4.013