Young-Jin Park1, Eun-Kyoung Kim1, Sook Moon1, Doo-Pyo Hong1, Jung Yoon Bae1, Jin Kim2. 1. Department of Oral Pathology, Oral Cancer Research Institute and Brain Korea PLUS 21 Project, College of Dentistry, Yonsei University, Seoul, Republic of Korea. 2. Department of Oral Pathology, Oral Cancer Research Institute and Brain Korea PLUS 21 Project, College of Dentistry, Yonsei University, Seoul, Republic of Korea jink@yuhs.ac.
Abstract
BACKGROUND/AIM: The present study aimed to investigate whether the down-regulation of human telomerase reverse transcriptase (hTERT) may induce an anti-invasive effect in oral squamous cell cancer cell lines. MATERIALS AND METHODS: A genetically-engineered squamous carcinoma cell line overexpressing hTERT in immortalized oral keratinocytes transfected by human papilloma virus (HPV)-16 E6/E7 (IHOK) was used. In vivo tumorigenicity was examined using an orthotopic xenograft model of nude mice. For evaluating anti-invasive activity by knockdown of hTERT expression, transwell invasion assay and real-time polymerase chain reaction (PCR) for matrix metalloproteinases (MMP) were employed. RESULTS: The down-regulation of hTERT expression reduced the invasive activity and MMP expression. This result was re-confirmed in the HSC3 oral squamous carcinoma cell line. CONCLUSION: Targeting hTERT may lead to novel therapeutic approaches. Copyright
BACKGROUND/AIM: The present study aimed to investigate whether the down-regulation of humantelomerase reverse transcriptase (hTERT) may induce an anti-invasive effect in oral squamous cell cancer cell lines. MATERIALS AND METHODS: A genetically-engineered squamous carcinoma cell line overexpressing hTERT in immortalized oral keratinocytes transfected by human papilloma virus (HPV)-16 E6/E7 (IHOK) was used. In vivo tumorigenicity was examined using an orthotopic xenograft model of nude mice. For evaluating anti-invasive activity by knockdown of hTERT expression, transwell invasion assay and real-time polymerase chain reaction (PCR) for matrix metalloproteinases (MMP) were employed. RESULTS: The down-regulation of hTERT expression reduced the invasive activity and MMP expression. This result was re-confirmed in the HSC3 oral squamous carcinoma cell line. CONCLUSION: Targeting hTERT may lead to novel therapeutic approaches. Copyright
Authors: Afza Ahmad; Rohit Kumar Tiwari; Prakriti Mishra; Ali G Alkhathami; Tahani M Almeleebia; Mohammad Y Alshahrani; Irfan Ahmad; Rawan Amer Asiri; Noura M Alabdullah; Mohamed Hussien; Mohd Saeed; Irfan Ahmad Ansari Journal: Saudi J Biol Sci Date: 2022-01-31 Impact factor: 4.052
Authors: Gennaro Altamura; Barbara Degli Uberti; Giorgio Galiero; Giovanna De Luca; Karen Power; Luca Licenziato; Paola Maiolino; Giuseppe Borzacchiello Journal: Front Vet Sci Date: 2021-01-20