Yumei Li1, Limei Yan2, Wenyu Zhang1, Nan Hu1, Wei Chen1, Hui Wang1, Min Kang3, Hesheng Ou1. 1. College of Pharmacy, Guangxi Medical University Nanning City, Guangxi Province, PR China. 2. Center of Biochemistry Research, University of South China Hengyang City, Hunan Province, PR China. 3. The First Affiliated Hospital of Guangxi Medical University Nanning City, Guangxi Province, PR China.
Abstract
OBJECTIVES: This study is to investigate whether microRNA (miR)-21 inhibits platelet-derived growth factor-induced human aortic vascular smooth muscle cell (VSMC) proliferation and migration through targeting activator protein-1 (AP-1). METHODS: VSMCs were transfected with the miR-21 or miR-21 inhibitor. Cell proliferation was determined using methyl thiazolyl tetrazolium assay. Cell migration was detected by transwell assay. Luciferase reporter assay was used to study the interaction between miR-21 and AP-1. The levels of mRNA were determined using quantitative real-time polymerase chain reaction, while protein expression was measured using Western blotting assay. RESULTS: Low expression of miR-21 significantly inhibited VSMC proliferation, invasion and migration. The mRNA levels and protein expression of α-SMA and AP-1 were down-regulated by low expression of miR-21. In addition, luciferase reporter assay demonstrated that AP-1 might be a direct target gene of miR-21 in VSMC initiation and development. Moreover, up-regulation of AP-1 was critical for miR-21-mediated inhibitory effects on platelet-derived growth factor-induced cell proliferation and migration in human VSMCs. CONCLUSIONS: In summary, miR-21 is a key molecule in regulating human VSMC proliferation and migration by targeting AP-1, suggesting that specific modulation of miR-21 in human VSMCs may become an attractive approach for the treatment of proliferative vascular diseases.
OBJECTIVES: This study is to investigate whether microRNA (miR)-21 inhibits platelet-derived growth factor-induced human aortic vascular smooth muscle cell (VSMC) proliferation and migration through targeting activator protein-1 (AP-1). METHODS: VSMCs were transfected with the miR-21 or miR-21 inhibitor. Cell proliferation was determined using methyl thiazolyl tetrazolium assay. Cell migration was detected by transwell assay. Luciferase reporter assay was used to study the interaction between miR-21 and AP-1. The levels of mRNA were determined using quantitative real-time polymerase chain reaction, while protein expression was measured using Western blotting assay. RESULTS: Low expression of miR-21 significantly inhibited VSMC proliferation, invasion and migration. The mRNA levels and protein expression of α-SMA and AP-1 were down-regulated by low expression of miR-21. In addition, luciferase reporter assay demonstrated that AP-1 might be a direct target gene of miR-21 in VSMC initiation and development. Moreover, up-regulation of AP-1 was critical for miR-21-mediated inhibitory effects on platelet-derived growth factor-induced cell proliferation and migration in human VSMCs. CONCLUSIONS: In summary, miR-21 is a key molecule in regulating human VSMC proliferation and migration by targeting AP-1, suggesting that specific modulation of miR-21 in human VSMCs may become an attractive approach for the treatment of proliferative vascular diseases.
Authors: Sebastian Albinsson; Yajaira Suarez; Athanasia Skoura; Stefan Offermanns; Joseph M Miano; William C Sessa Journal: Arterioscler Thromb Vasc Biol Date: 2010-04-08 Impact factor: 8.311
Authors: M Tsujiura; D Ichikawa; S Komatsu; A Shiozaki; H Takeshita; T Kosuga; H Konishi; R Morimura; K Deguchi; H Fujiwara; K Okamoto; E Otsuji Journal: Br J Cancer Date: 2010-03-16 Impact factor: 7.640
Authors: Caifu Chen; Dana A Ridzon; Adam J Broomer; Zhaohui Zhou; Danny H Lee; Julie T Nguyen; Maura Barbisin; Nan Lan Xu; Vikram R Mahuvakar; Mark R Andersen; Kai Qin Lao; Kenneth J Livak; Karl J Guegler Journal: Nucleic Acids Res Date: 2005-11-27 Impact factor: 16.971
Authors: Johanna Christine Jansing; Jan Fiedler; Andreas Pich; Janika Viereck; Thomas Thum; Christian Mühlfeld; Christina Brandenberger Journal: Int J Mol Sci Date: 2020-01-27 Impact factor: 5.923
Authors: Beate Hoppe; Stefan Pietsch; Martin Franke; Sven Engel; Marco Groth; Matthias Platzer; Christoph Englert Journal: BMC Dev Biol Date: 2015-11-17 Impact factor: 1.978