Literature DB >> 25358776

Determining the role of metal binding in protein cage assembly.

Anne Grove1, Ambuj K Kushwaha, Khoa H Nguyen.   

Abstract

Assembly of protein cages may require structural metal ions to nucleate or stabilize association of protein subunits. We describe here an approach to establishing the role of metal ions in protein cage assembly and stability, focusing on detecting the presence of structural metal ions as well as establishing oligomeric state. A colorimetric assay for detection of metal is described, along with a gel filtration assay to assess protein cage assembly and a fluorescence-based method for determining protein stability.

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Year:  2015        PMID： 25358776     DOI： 10.1007/978-1-4939-2131-7_9

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  4 in total

1.  Redox-Sensitive MarR Homologue BifR from Burkholderia thailandensis Regulates Biofilm Formation.

Authors:  Ashish Gupta; Stanley M Fuentes; Anne Grove
Journal:  Biochemistry       Date:  2017-04-21       Impact factor: 3.162

2.  Gene Regulation by Redox-Sensitive Burkholderia thailandensis OhrR and Its Role in Bacterial Killing of Caenorhabditis elegans.

Authors:  Anuja Pande; Todd C Veale; Anne Grove
Journal:  Infect Immun       Date:  2018-08-22       Impact factor: 3.441

3.  Similar solutions to a common challenge: regulation of genes encoding Ralstonia solanacearum xanthine dehydrogenase.

Authors:  Smitha Sivapragasam; Arpita Ghosh; Sanjay Kumar; Danté T Johnson; Anne Grove
Journal:  FEMS Microbiol Lett       Date:  2021-03-03       Impact factor: 2.742

4.  Gel-Filtration Chromatography.

Authors:  Ciarán Ó'Fágáin; Philip M Cummins; Brendan F O'Connor
Journal:  Methods Mol Biol       Date:  2017
  4 in total

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