Li Gao1, You-Qiang Fang2, Tian-Yu Zhang1, Bo Ge1, Bin Xu1, Jie-Fu Huang1, Zhen-Feng Zhang3, Ning Tan4. 1. Department of Urology, Affiliated Hospital, Guilin Medical College Guilin 541004, Guangxi, China. 2. Department of Urology, The Third Affiliated Hospital, Sun Yat-Sen University Guangzhou 510630, China. 3. State Key Laboratory of Oncogenes and Related Genes Shanghai Cancer Institute, Shanghai 200032, China. 4. Center of Science Research, Guilin Medical College Guilin 541004, Guangxi, China.
Abstract
OBJECTIVE: GSTP1 over-expression was introduced into human bladder cancer T24 cells via the lentivirus system. The influence of GSTP1 on the proliferation and cell cycle of T24 cells as well as the potential mechanisms was investigated. METHODS: The lentiviral vector GSTP1-pWPXL was constructed and transfected into T24 cells in the presence of Lipofectamine 2000. CCK8 assay and colony formation test were performed to explore the impact of GSTP1 on the proliferation of T24 cells. Ollowing PI staining, flow cytometry was done to detect the proportion of T24 cells in different phases. Western blot assay was conducted to detect the protein p21 expression. RESULTS: When compared with control group, T24 cells with GSTP1 over-expression showed significant reduction in cell proliferation (P < 0.01) and they were arrested in G0/G1 phase. Western blot assay indicated that the p21 protein expression in T24 cells with GSTP1 over-expression was significantly higher than that in control group. CONCLUSION: GSTP1 may inhibit the proliferation of T24 cells and arrest these cells in G0/G1 phase, which may be ascribed to the up-regulated expression of p21.
OBJECTIVE:GSTP1 over-expression was introduced into humanbladder cancer T24 cells via the lentivirus system. The influence of GSTP1 on the proliferation and cell cycle of T24 cells as well as the potential mechanisms was investigated. METHODS: The lentiviral vector GSTP1-pWPXL was constructed and transfected into T24 cells in the presence of Lipofectamine 2000. CCK8 assay and colony formation test were performed to explore the impact of GSTP1 on the proliferation of T24 cells. Ollowing PI staining, flow cytometry was done to detect the proportion of T24 cells in different phases. Western blot assay was conducted to detect the protein p21 expression. RESULTS: When compared with control group, T24 cells with GSTP1 over-expression showed significant reduction in cell proliferation (P < 0.01) and they were arrested in G0/G1 phase. Western blot assay indicated that the p21 protein expression in T24 cells with GSTP1 over-expression was significantly higher than that in control group. CONCLUSION:GSTP1 may inhibit the proliferation of T24 cells and arrest these cells in G0/G1 phase, which may be ascribed to the up-regulated expression of p21.