| Literature DB >> 2535 |
Abstract
The microsomal 3-hydroxysteroid dehydrogenases were solubilized with lubrol, a non-ionic detergent. A 3alpha-hydroxysteroid dehydrogenase is purified about 100-fold by double affinity chromatography on 5alpha-dihydrotestosterone-Sepharose. This enzyme can use both NADH and NADPH as coenzymes.Entities:
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Year: 1976 PMID： 2535 DOI： 10.1515/bchm2.1976.357.1.35
Source DB: PubMed Journal: Hoppe Seylers Z Physiol Chem ISSN： 0018-4888