OBJECTIVE: To find out whether gDNA methylation can be used as a diagnostic/prognostic method for neonatal sepsis. METHODS: The study was conducted in the neonatal division of a tertiary care referral hospital. Fifty one newborns as cases and thirty seven newborns as controls were enrolled in the study. Using 5-mC DNA ELISA method, the percentage of genomic DNA methylated in these newborns was established. RESULTS: Highly significant difference in percentage of gDNA methylated was found between the cases and controls (Cases: 2.4 ± 0.39; CONTROLS: 2.07 ± 0.35; P < 0.0001). Culture proven and possible cases were also significantly distinguishable (P < 0.05). No significant differences in methylation were observed in terms of gestational age, birth weight and outcomes such shock, thrombocytopenia, except for renal failure. CONCLUSIONS: The index results showed that genomic DNA methylation varies significantly among newborns with sepsis (clinical, probable and culture positive) and without sepsis. Although the global DNA methylation was not a highly sensitive diagnostic method, this study reveals that DNA methylation might play a vital role in neonatal sepsis susceptibility. Identification of the specific differentially methylated genes might serve as a promising future diagnostic/prognostic marker for neonatal sepsis.
OBJECTIVE: To find out whether gDNA methylation can be used as a diagnostic/prognostic method for neonatal sepsis. METHODS: The study was conducted in the neonatal division of a tertiary care referral hospital. Fifty one newborns as cases and thirty seven newborns as controls were enrolled in the study. Using 5-mC DNA ELISA method, the percentage of genomic DNA methylated in these newborns was established. RESULTS: Highly significant difference in percentage of gDNA methylated was found between the cases and controls (Cases: 2.4 ± 0.39; CONTROLS: 2.07 ± 0.35; P < 0.0001). Culture proven and possible cases were also significantly distinguishable (P < 0.05). No significant differences in methylation were observed in terms of gestational age, birth weight and outcomes such shock, thrombocytopenia, except for renal failure. CONCLUSIONS: The index results showed that genomic DNA methylation varies significantly among newborns with sepsis (clinical, probable and culture positive) and without sepsis. Although the global DNA methylation was not a highly sensitive diagnostic method, this study reveals that DNA methylation might play a vital role in neonatal sepsis susceptibility. Identification of the specific differentially methylated genes might serve as a promising future diagnostic/prognostic marker for neonatal sepsis.
Authors: Benjamin Goeppert; Carolin Konermann; Christopher Roman Schmidt; Olga Bogatyrova; Lea Geiselhart; Christina Ernst; Lei Gu; Natalia Becker; Manuela Zucknick; Arianeb Mehrabi; Mohammadreza Hafezi; Frederick Klauschen; Albrecht Stenzinger; Arne Warth; Kai Breuhahn; Marcus Renner; Wilko Weichert; Peter Schirmacher; Christoph Plass; Dieter Weichenhan Journal: Hepatology Date: 2013-12-24 Impact factor: 17.425
Authors: Kristina A Tendl; Stefan M F Schulz; Thomas P Mechtler; Adele Bohn; Thomas Metz; Susanne Greber-Platzer; David C Kasper; Kurt R Herkner; Chike B Item Journal: Epigenetics Date: 2013-10-17 Impact factor: 4.528
Authors: E Scarr; J M Craig; M J Cairns; M S Seo; J C Galati; N J Beveridge; A Gibbons; S Juzva; B Weinrich; M Parkinson-Bates; A P Carroll; R Saffery; B Dean Journal: Transl Psychiatry Date: 2013-02-19 Impact factor: 6.222
Authors: F Guénard; L Bouchard; A Tchernof; Y Deshaies; F S Hould; S Lebel; P Marceau; L Pérusse; M C Vohl Journal: Int J Genomics Date: 2013-08-06 Impact factor: 2.326
Authors: Reinhold Wasserkort; Alexandra Kalmar; Gabor Valcz; Sandor Spisak; Manuel Krispin; Kinga Toth; Zsolt Tulassay; Andrew Z Sledziewski; Bela Molnar Journal: BMC Cancer Date: 2013-08-30 Impact factor: 4.430
Authors: Fredrick J Bohanon; Amy A Mrazek; Mohamed T Shabana; Sarah Mims; Geetha L Radhakrishnan; George C Kramer; Ravi S Radhakrishnan Journal: Am J Surg Date: 2015-06-26 Impact factor: 2.565
Authors: Clifford S Deutschman; Judith Hellman; Ricard Ferrer Roca; Daniel De Backer; Craig M Coopersmith Journal: Crit Care Med Date: 2020-08 Impact factor: 9.296