Literature DB >> 25348177

Triple resonance-based ¹³C(α) and ¹³C(β) CEST experiments for studies of ms timescale dynamics in proteins.

Dong Long1, Ashok Sekhar, Lewis E Kay.   

Abstract

A pair of triple resonance based CEST pulse schemes are presented for measuring ¹³C(α) and ¹³C(β) chemical shifts of sparsely populated and transiently formed conformers that are invisible to traditional NMR experiments. CEST profiles containing dips at resonance positions of ¹³C(α) or ¹³C(β) spins of major (ground) and minor (excited) conformers are obtained in a pseudo 3rd dimension that is generated by quantifying modulations of cross peaks in ¹⁵N, ¹H(N) correlation spectra. An application to the folding reaction of a G48A mutant of the Fyn SH3 domain is presented, illustrating and validating the methodology.

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Year:  2014        PMID： 25348177     DOI： 10.1007/s10858-014-9868-5

Source DB:  PubMed          Journal:  J Biomol NMR        ISSN： 0925-2738            Impact factor:   2.835


  11 in total

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Authors:  Lewis E Kay; Mitsuhiko Ikura; Rolf Tschudin; Ad Bax
Journal:  J Magn Reson       Date:  2011-12       Impact factor: 2.229

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3.  Studying "invisible" excited protein states in slow exchange with a major state conformation.

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4.  Visualizing side chains of invisible protein conformers by solution NMR.

Authors:  Guillaume Bouvignies; Pramodh Vallurupalli; Lewis E Kay
Journal:  J Mol Biol       Date:  2013-11-08       Impact factor: 5.469

5.  SHIFTX2: significantly improved protein chemical shift prediction.

Authors:  Beomsoo Han; Yifeng Liu; Simon W Ginzinger; David S Wishart
Journal:  J Biomol NMR       Date:  2011-03-30       Impact factor: 2.835

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Journal:  J Biomol NMR       Date:  1993-03       Impact factor: 2.835

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Authors:  T M Logan; E T Olejniczak; R X Xu; S W Fesik
Journal:  J Biomol NMR       Date:  1993-03       Impact factor: 2.835

8.  The 13C chemical-shift index: a simple method for the identification of protein secondary structure using 13C chemical-shift data.

Authors:  D S Wishart; B D Sykes
Journal:  J Biomol NMR       Date:  1994-03       Impact factor: 2.835

9.  Measuring hydrogen exchange rates in invisible protein excited states.

Authors:  Dong Long; Guillaume Bouvignies; Lewis E Kay
Journal:  Proc Natl Acad Sci U S A       Date:  2014-06-02       Impact factor: 11.205

10.  TALOS+: a hybrid method for predicting protein backbone torsion angles from NMR chemical shifts.

Authors:  Yang Shen; Frank Delaglio; Gabriel Cornilescu; Ad Bax
Journal:  J Biomol NMR       Date:  2009-06-23       Impact factor: 2.835
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  10 in total

1.  Mapping the conformation of a client protein through the Hsp70 functional cycle.

Authors:  Ashok Sekhar; Rina Rosenzweig; Guillaume Bouvignies; Lewis E Kay
Journal:  Proc Natl Acad Sci U S A       Date:  2015-08-03       Impact factor: 11.205

2.  Probing the excited-state chemical shifts and exchange parameters by nitrogen-decoupled amide proton chemical exchange saturation transfer (HNdec-CEST).

Authors:  Qinglin Wu; Benjamin A Fenton; Jessica L Wojtaszek; Pei Zhou
Journal:  Chem Commun (Camb)       Date:  2017-07-27       Impact factor: 6.222

Review 3.  Probing conformational dynamics in biomolecules via chemical exchange saturation transfer: a primer.

Authors:  Pramodh Vallurupalli; Ashok Sekhar; Tairan Yuwen; Lewis E Kay
Journal:  J Biomol NMR       Date:  2017-03-19       Impact factor: 2.835

4.  Hsp70 biases the folding pathways of client proteins.

Authors:  Ashok Sekhar; Rina Rosenzweig; Guillaume Bouvignies; Lewis E Kay
Journal:  Proc Natl Acad Sci U S A       Date:  2016-05-02       Impact factor: 11.205

5.  Measuring Residual Dipolar Couplings in Excited Conformational States of Nucleic Acids by CEST NMR Spectroscopy.

Authors:  Bo Zhao; Qi Zhang
Journal:  J Am Chem Soc       Date:  2015-10-15       Impact factor: 15.419

6.  Thermal fluctuations of immature SOD1 lead to separate folding and misfolding pathways.

Authors:  Ashok Sekhar; Jessica A O Rumfeldt; Helen R Broom; Colleen M Doyle; Guillaume Bouvignies; Elizabeth M Meiering; Lewis E Kay
Journal:  Elife       Date:  2015-06-23       Impact factor: 8.140

Review 7.  NMR methods for exploring 'dark' states in ligand binding and protein-protein interactions.

Authors:  Vitali Tugarinov; Alberto Ceccon; G Marius Clore
Journal:  Prog Nucl Magn Reson Spectrosc       Date:  2021-11-02       Impact factor: 9.795

8.  The A39G FF domain folds on a volcano-shaped free energy surface via separate pathways.

Authors:  Ved P Tiwari; Yuki Toyama; Debajyoti De; Lewis E Kay; Pramodh Vallurupalli
Journal:  Proc Natl Acad Sci U S A       Date:  2021-11-16       Impact factor: 11.205

9.  Interaction of Huntingtin Exon-1 Peptides with Lipid-Based Micellar Nanoparticles Probed by Solution NMR and Q-Band Pulsed EPR.

Authors:  Alberto Ceccon; Thomas Schmidt; Vitali Tugarinov; Samuel A Kotler; Charles D Schwieters; G Marius Clore
Journal:  J Am Chem Soc       Date:  2018-05-14       Impact factor: 15.419

10.  Measuring radiofrequency fields in NMR spectroscopy using offset-dependent nutation profiles.

Authors:  Ahallya Jaladeep; Claris Niya Varghese; Ashok Sekhar
Journal:  J Magn Reson       Date:  2021-07-06       Impact factor: 2.734
  10 in total

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