Literature DB >> 25340456

Investigating fluorescent dyes in fluorescence-assisted screenings.

Joo-Eun Jee1, Jaehong Lim, Hoon Hyun, Jessica Oon, Yong Siang Ong, Cedrik Massif, Young-Tae Chang, Hak Soo Choi, Su Seong Lee.   

Abstract

Screening of bead-based peptide libraries against fluorescent dye-labeled target proteins was found to be significantly influenced by the dye characteristics. Commercially available red fluorescent dyes with net negative charges adversely showed strong interactions with library beads. The introduction of zwitterionic dyes significantly reduced the unwanted interactions, which sheds light upon using the right fluorescent probe for acquisition of reliable results in various fluorescence-assisted applications.

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Year:  2014        PMID： 25340456      PMCID： PMC4224607          DOI： 10.1039/c4cc07008a

Source DB:  PubMed          Journal:  Chem Commun (Camb)        ISSN： 1359-7345            Impact factor:   6.222


  22 in total

1.  Optimized protocols for the isolation of specific protein-binding peptides or peptoids from combinatorial libraries displayed on beads.

Authors:  Thomas Kodadek; Kiran Bachhawat-Sikder
Journal:  Mol Biosyst       Date:  2005-11-28

2.  High-throughput screening of one-bead-one-compound peptide libraries using intact cells.

Authors:  Choi-Fong Cho; Babak Behnam Azad; Leonard G Luyt; John D Lewis
Journal:  ACS Comb Sci       Date:  2013-07-19       Impact factor: 3.784

3.  Single bead labeling method for combining confocal fluorescence on-bead screening and solution validation of tagged one-bead one-compound libraries.

Authors:  Martin Hintersteiner; Thierry Kimmerlin; Frank Kalthoff; Markus Stoeckli; Geraldine Garavel; Jan-Marcus Seifert; Nicole-Claudia Meisner; Volker Uhl; Christof Buehler; Thomas Weidemann; Manfred Auer
Journal:  Chem Biol       Date:  2009-07-31

4.  Iterative in situ click chemistry assembles a branched capture agent and allosteric inhibitor for Akt1.

Authors:  Steven W Millward; Ryan K Henning; Gabriel A Kwong; Suresh Pitram; Heather D Agnew; Kaycie M Deyle; Arundhati Nag; Jason Hein; Su Seong Lee; Jaehong Lim; Jessica A Pfeilsticker; K Barry Sharpless; James R Heath
Journal:  J Am Chem Soc       Date:  2011-10-24       Impact factor: 15.419

5.  Process automation toward ultra-high-throughput screening of combinatorial one-bead-one-compound (OBOC) peptide libraries.

Authors:  Junhoe Cha; Jaehong Lim; Yiran Zheng; Sylvia Tan; Yi Li Ang; Jessica Oon; Mei Wei Ang; Jingjing Ling; Marcus Bode; Su Seong Lee
Journal:  J Lab Autom       Date:  2012-02-14

6.  Effects of surface charge on denaturation of bovine carbonic anhydrase.

Authors:  Irina Gitlin; Katherine L Gudiksen; George M Whitesides
Journal:  Chembiochem       Date:  2006-08       Impact factor: 3.164

7.  Screening of one-bead-one-peptide combinatorial library using red fluorescent dyes. Presence of positive and false positive beads.

Authors:  Mariela M Marani; María C Martínez Ceron; Silvana L Giudicessi; Eliandre de Oliveira; Simon Côté; Rosa Erra-Balsells; Fernando Albericio; Osvaldo Cascone; Silvia A Camperi
Journal:  J Comb Chem       Date:  2009 Jan-Feb

8.  Synthesis and screening of a cyclic peptide library: discovery of small-molecule ligands against human prolactin receptor.

Authors:  Tao Liu; Sang Hoon Joo; Jeffrey L Voorhees; Charles L Brooks; Dehua Pei
Journal:  Bioorg Med Chem       Date:  2008-01-13       Impact factor: 3.641

9.  Synthesis and screening of support-bound combinatorial peptide libraries with free C-termini: determination of the sequence specificity of PDZ domains.

Authors:  Sang Hoon Joo; Dehua Pei
Journal:  Biochemistry       Date:  2008-01-31       Impact factor: 3.162

10.  Iterative in situ click chemistry creates antibody-like protein-capture agents.

Authors:  Heather D Agnew; Rosemary D Rohde; Steven W Millward; Arundhati Nag; Woon-Seok Yeo; Jason E Hein; Suresh M Pitram; Abdul Ahad Tariq; Vanessa M Burns; Russell J Krom; Valery V Fokin; K Barry Sharpless; James R Heath
Journal:  Angew Chem Int Ed Engl       Date:  2009       Impact factor: 15.336
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  2 in total

1.  Xenoprotein engineering via synthetic libraries.

Authors:  Zachary P Gates; Alexander A Vinogradov; Anthony J Quartararo; Anupam Bandyopadhyay; Zi-Ning Choo; Ethan D Evans; Kathryn H Halloran; Alexander J Mijalis; Surin K Mong; Mark D Simon; Eric A Standley; Evan D Styduhar; Sarah Z Tasker; Faycal Touti; Jessica M Weber; Jessica L Wilson; Timothy F Jamison; Bradley L Pentelute
Journal:  Proc Natl Acad Sci U S A       Date:  2018-05-21       Impact factor: 11.205

2.  An efficient strategy to enhance binding affinity and specificity of a known isozyme inhibitor.

Authors:  Joo-Eun Jee; Jaehong Lim; Yong Siang Ong; Jessica Oon; Liqian Gao; Hak Soo Choi; Su Seong Lee
Journal:  Org Biomol Chem       Date:  2016-07-12       Impact factor: 3.876
  2 in total

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