Antônio Frederico Freitas Gomides1, Sérgio Oliveira de Paula2, Reggiani Vilela Gonçalves3, Leandro Licursi de Oliveira4, Célia Lúcia de Luces Fortes Ferreira5, Débora Silva Comastri6, Maria do Carmo Gouveia Peluzio7. 1. Department of General Biology. Federal University of Viçosa, MG. Brazil. fredaogomides@yahoo.com.br. 2. Department of General Biology. Federal University of Viçosa, MG. Brazil. oliveira@yahoo.com.br. 3. Department of Animal Biology. Federal University of Viçosa, MG. Brazil. 4. Department of General Biology. Federal University of Viçosa, MG. Brazil. llicursi@yahoo.com.br. 5. Department of General Biology. Federal University of Viçosa, MG. Brazil. clferreira@yahoo.com.br. 6. Department of Food Technology. Federal University of Viçosa, MG. Brazil. chaves@yahoo.com.br. 7. Department of Nutrition and Health. Federal University of Viçosa, MG. Brazil. mpeluzio@ufv.br.
Abstract
UNLABELLED: Studies have shown fibers to be effective in reducing the appearance of aberrant crypt foci (ACF) in rodents. OBJECTIVE: The goal of this study was to investigate the preventive effects of fructooligosaccharide (FOS) and inulin prebiotics on the appearance of ACF in mice. MATERIALS AND METHODS: The techniques used were: RT-PCR to evaluate the gene expression of p16, p21, p54, cyclin D1 and cyclin E in the distal colon; the quantification of Number of aberrant crypt foci (ACF) and measurement of catalase activity in the liver and distal colon. The animals were divided into five treatments (n=8); C-: AIN93M diet without fibers + DMH (1,2-dimethylhydrazine); INL: AIN93M diet with inulin; INLCA: AIN93M diet with inulin + DMH; FOS: AIN93M diet with FOS; FOSCA: AIN93M diet with FOS + DMH, during 15 weeks. RESULTS: Inulin prevented the appearance of ACF in the proximal, middle and distal colon, compared to the control without fibers. In the middle and distal colon, FOS was also effective in preventing the incidence of ACF. This effectiveness may be attributed to the increased gene expression of p16 following FOS treatment. Both prebiotics also decreased catalase activity in the distal colon, thus suggesting an antioxidant effect. CONCLUSION: These results suggesting an antioxidant effect prebiotics that may be attributed to the increased gene expression of p16. Copyright AULA MEDICA EDICIONES 2014. Published by AULA MEDICA. All rights reserved.
UNLABELLED: Studies have shown fibers to be effective in reducing the appearance of aberrant crypt foci (ACF) in rodents. OBJECTIVE: The goal of this study was to investigate the preventive effects of fructooligosaccharide (FOS) and inulin prebiotics on the appearance of ACF in mice. MATERIALS AND METHODS: The techniques used were: RT-PCR to evaluate the gene expression of p16, p21, p54, cyclin D1 and cyclin E in the distal colon; the quantification of Number of aberrant crypt foci (ACF) and measurement of catalase activity in the liver and distal colon. The animals were divided into five treatments (n=8); C-: AIN93M diet without fibers + DMH (1,2-dimethylhydrazine); INL: AIN93M diet with inulin; INLCA: AIN93M diet with inulin + DMH; FOS: AIN93M diet with FOS; FOSCA: AIN93M diet with FOS + DMH, during 15 weeks. RESULTS: Inulin prevented the appearance of ACF in the proximal, middle and distal colon, compared to the control without fibers. In the middle and distal colon, FOS was also effective in preventing the incidence of ACF. This effectiveness may be attributed to the increased gene expression of p16 following FOS treatment. Both prebiotics also decreased catalase activity in the distal colon, thus suggesting an antioxidant effect. CONCLUSION: These results suggesting an antioxidant effect prebiotics that may be attributed to the increased gene expression of p16. Copyright AULA MEDICA EDICIONES 2014. Published by AULA MEDICA. All rights reserved.