Literature DB >> 25332757

Six consecutive false positive cases from cell-free fetal DNA testing in a single referring centre.

Nella Dugo1, Francesco Padula1, Luisa Mobili1, Cristiana Brizzi1, Laura D'Emidio1, Pietro Cignini1, Alvaro Mesoraca2, Domenico Bizzoco2, Antonella Cima2, Claudio Giorlandino1.   

Abstract

INTRODUCTION: recent studies have proposed the introduction of cell-free fetal DNA testing (NIPT-Non Invasive Prenatal Testing) in routine clinical practice emphasizing its high sensibility and specificity. In any case, false positive and false negative findings may result from placental mosaicism, because cell-free fetal DNA originates mainly from placenta. CASE: WE REPORT SIX CASES OF WOMEN WHO UNDERWENT CHORIONIC VILLUS SAMPLING (CVS) OR AMNIOCENTESIS TO CONFIRM THE RESULTS FROM NIPT: two Turner syndromes, two Triple X, one Patau syndrome, one Edward syndrome.
RESULTS: using classic cytogenetic analysis and, also, Array - Comparative Genomic Hybridization (Array CGH) the karyotype of all 5 fetuses was found to be normal.
CONCLUSION: results from NIPT must always be confirmed by invasive prenatal diagnosis. It is mandatory to inform the patient that the CVS and amniocentesis still represent the only form of prenatal diagnostic test available.

Entities:  

Keywords:  NIPT; amniocentesis; aneuploidy; cell-free fetal DNA; chorionic villus sampling

Year:  2014        PMID: 25332757      PMCID: PMC4187000     

Source DB:  PubMed          Journal:  J Prenat Med        ISSN: 1971-3282


  30 in total

1.  Antibiotic prophylaxis before second-trimester genetic amniocentesis (APGA): a single-centre open randomised controlled trial.

Authors:  Claudio Giorlandino; Pietro Cignini; Marco Cini; Cristiana Brizzi; Ornella Carcioppolo; Vincenzo Milite; Claudio Coco; Paolo Gentili; Lucia Mangiafico; Alvaro Mesoraca; Domenico Bizzoco; Ivan Gabrielli; Luisa Mobili
Journal:  Prenat Diagn       Date:  2009-06       Impact factor: 3.050

Review 2.  Non-invasive prenatal testing for fetal chromosomal abnormalities by low-coverage whole-genome sequencing of maternal plasma DNA: review of 1982 consecutive cases in a single center.

Authors:  T K Lau; S W Cheung; P S S Lo; A N Pursley; M K Chan; F Jiang; H Zhang; W Wang; L F J Jong; O K C Yuen; H Y C Chan; W S K Chan; K W Choy
Journal:  Ultrasound Obstet Gynecol       Date:  2014-02-10       Impact factor: 7.299

3.  Position Statement from the Italian College of Fetal Maternal Medicine: Non-invasive prenatal testing (NIPT) by maternal plasma DNA sequencing.

Authors: 
Journal:  J Prenat Med       Date:  2013-04

Review 4.  Cell-free fetal DNA and RNA in maternal blood: implications for safer antenatal testing.

Authors:  Caroline F Wright; Lyn S Chitty
Journal:  BMJ       Date:  2009-07-06

5.  Noninvasive prenatal detection of fetal trisomy 18 by epigenetic allelic ratio analysis in maternal plasma: Theoretical and empirical considerations.

Authors:  Yu K Tong; Chunming Ding; Rossa W K Chiu; Ageliki Gerovassili; Stephen S C Chim; Tak Y Leung; Tse N Leung; Tze K Lau; Kypros H Nicolaides; Y M Dennis Lo
Journal:  Clin Chem       Date:  2006-10-13       Impact factor: 8.327

6.  Non-invasive prenatal assessment of trisomy 21 by multiplexed maternal plasma DNA sequencing: large scale validity study.

Authors:  Rossa W K Chiu; Ranjit Akolekar; Yama W L Zheng; Tak Y Leung; Hao Sun; K C Allen Chan; Fiona M F Lun; Attie T J I Go; Elizabeth T Lau; William W K To; Wing C Leung; Rebecca Y K Tang; Sidney K C Au-Yeung; Helena Lam; Yu Y Kung; Xiuqing Zhang; John M G van Vugt; Ryoko Minekawa; Mary H Y Tang; Jun Wang; Cees B M Oudejans; Tze K Lau; Kypros H Nicolaides; Y M Dennis Lo
Journal:  BMJ       Date:  2011-01-11

7.  Maternal mosaicism is a significant contributor to discordant sex chromosomal aneuploidies associated with noninvasive prenatal testing.

Authors:  Yanlin Wang; Yan Chen; Feng Tian; Jianguang Zhang; Zhuo Song; Yi Wu; Xu Han; Wenjing Hu; Duan Ma; David Cram; Weiwei Cheng
Journal:  Clin Chem       Date:  2013-11-05       Impact factor: 8.327

Review 8.  Cell-free fetal DNA and intact fetal cells in maternal blood circulation: implications for first and second trimester non-invasive prenatal diagnosis.

Authors:  Farideh Z Bischoff; Mina K Sinacori; Dianne D Dang; Deborah Marquez-Do; Cassandra Horne; Dorothy E Lewis; Joe Leigh Simpson
Journal:  Hum Reprod Update       Date:  2002 Nov-Dec       Impact factor: 15.610

9.  Effect of high throughput RHD typing of fetal DNA in maternal plasma on use of anti-RhD immunoglobulin in RhD negative pregnant women: prospective feasibility study.

Authors:  Kirstin Finning; Pete Martin; Joanna Summers; Edwin Massey; Geoff Poole; Geoff Daniels
Journal:  BMJ       Date:  2008-04-03

10.  Incorporating DNA sequencing into current prenatal screening practice for Down's syndrome.

Authors:  Nicholas J Wald; Jonathan P Bestwick
Journal:  PLoS One       Date:  2013-03-20       Impact factor: 3.240

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  5 in total

1.  Non-invasive prenatal testing for sex chromosome abnormalities: a source of confusion.

Authors:  Erkan Kalafat; Mehmet Murat Seval; Batuhan Turgay; Acar Koç
Journal:  BMJ Case Rep       Date:  2015-01-28

2.  Biological explanations for discordant noninvasive prenatal test results: Preliminary data and lessons learned.

Authors:  Louise Wilkins-Haug; Chengsheng Zhang; Eliza Cerveira; Mallory Ryan; Adam Mil-Homens; Qihui Zhu; Honey Reddi; Charles Lee; Diana W Bianchi
Journal:  Prenat Diagn       Date:  2018-05       Impact factor: 3.050

3.  Obstetric professionals' perceptions of non-invasive prenatal testing for Down syndrome: clinical usefulness compared with existing tests and ethical implications.

Authors:  Olivia Miu Yung Ngan; Huso Yi; Samuel Yeung Shan Wong; Daljit Sahota; Shenaz Ahmed
Journal:  BMC Pregnancy Childbirth       Date:  2017-09-05       Impact factor: 3.007

4.  Incidental finding of maternal malignancy in an unusual non-invasive prenatal test and a review of similar cases.

Authors:  Maria Hammer Moellgaard; Ida Charlotte Bay Lund; Naja Becher; Anne-Bine Skytte; Lotte Andreasen; Malgorzata Ilona Srebniak; Ida Vogel
Journal:  Clin Case Rep       Date:  2022-10-11

Review 5.  Cell-Free DNA for the Management of Classical Hodgkin Lymphoma.

Authors:  Vincent Camus; Fabrice Jardin
Journal:  Pharmaceuticals (Basel)       Date:  2021-03-02
  5 in total

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