Literature DB >> 25331141

The Laurdan spectral phasor method to explore membrane micro-heterogeneity and lipid domains in live cells.

Ottavia Golfetto1, Elizabeth Hinde, Enrico Gratton.   

Abstract

In this method paper we describe the spectral phasor analysis applied to Laurdan emission for the assessment of the fluidity of different membranes in live cells. We first introduce the general context and then we show how to obtain the spectral phasor from data acquired using a commercial microscope.

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Year:  2015        PMID： 25331141     DOI： 10.1007/978-1-4939-1752-5_19

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  11 in total

1.  Spectral phasor analysis of LAURDAN fluorescence in live A549 lung cells to study the hydration and time evolution of intracellular lamellar body-like structures.

Authors:  Leonel Malacrida; Soledad Astrada; Arturo Briva; Mariela Bollati-Fogolín; Enrico Gratton; Luis A Bagatolli
Journal:  Biochim Biophys Acta       Date:  2016-07-30

2.  Noninvasive two-photon optical biopsy of retinal fluorophores.

Authors:  Grazyna Palczewska; Jakub Boguslawski; Patrycjusz Stremplewski; Lukasz Kornaszewski; Jianye Zhang; Zhiqian Dong; Xiao-Xuan Liang; Enrico Gratton; Alfred Vogel; Maciej Wojtkowski; Krzysztof Palczewski
Journal:  Proc Natl Acad Sci U S A       Date:  2020-08-26       Impact factor: 11.205

3.  New insight into the interaction of TRAF2 C-terminal domain with lipid raft microdomains.

Authors:  Arianna Ceccarelli; Almerinda Di Venere; Eleonora Nicolai; Anastasia De Luca; Nicola Rosato; Enrico Gratton; Giampiero Mei; Anna Maria Caccuri
Journal:  Biochim Biophys Acta Mol Cell Biol Lipids       Date:  2017-05-09       Impact factor: 4.698

Review 4.  Visualizing the regulation of SLC34 proteins at the apical membrane.

Authors:  Moshe Levi; Enrico Gratton
Journal:  Pflugers Arch       Date:  2019-01-06       Impact factor: 3.657

5.  Spectral Phasor Analysis of Nile Red Identifies Membrane Microenvironment Changes in the Presence of Amyloid Peptides.

Authors:  Bhawantha M Jayawardena; Resmi Menon; Mark R Jones; Christopher E Jones
Journal:  Cell Biochem Biophys       Date:  2022-10-06       Impact factor: 2.989

6.  Effect of osmotic stress on live cell plasma membranes, probed via Laurdan general polarization measurements.

Authors:  Elmer Zapata-Mercado; Evgenia V Azarova; Kalina Hristova
Journal:  Biophys J       Date:  2022-05-19       Impact factor: 3.699

7.  Model-free methods to study membrane environmental probes: a comparison of the spectral phasor and generalized polarization approaches.

Authors:  Leonel Malacrida; Enrico Gratton; David M Jameson
Journal:  Methods Appl Fluoresc       Date:  2015-11-12       Impact factor: 3.009

8.  Spectral imaging to measure heterogeneity in membrane lipid packing.

Authors:  Erdinc Sezgin; Dominic Waithe; Jorge Bernardino de la Serna; Christian Eggeling
Journal:  Chemphyschem       Date:  2015-03-05       Impact factor: 3.102

Review 9.  Interleaflet Coupling, Pinning, and Leaflet Asymmetry-Major Players in Plasma Membrane Nanodomain Formation.

Authors:  Toyoshi Fujimoto; Ingela Parmryd
Journal:  Front Cell Dev Biol       Date:  2017-01-10

10.  Identification of microplastics using 4-dimethylamino-4'-nitrostilbene solvatochromic fluorescence.

Authors:  Giuseppe Sancataldo; Vittorio Ferrara; Francesco Paolo Bonomo; Delia Francesca Chillura Martino; Mariano Licciardi; Bruno Giuseppe Pignataro; Valeria Vetri
Journal:  Microsc Res Tech       Date:  2021-05-28       Impact factor: 2.893
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