Zhenya Ivanova1, Bodil Bjørndal2, Natalia Grigorova1, Anton Roussenov3, Ekaterina Vachkova1, Kjetil Berge4, Lena Burri4, Rolf Berge2, Spaska Stanilova5, Anelia Milanova1, Georgi Penchev6, Rita Vik2, Vladimir Petrov7, Teodora Mircheva Georgieva1, Boycho Bivolraski1, Ivan Penchev Georgiev8. 1. Department of Pharmacology, Animal Physiology and Physiological Chemistry, Faculty of Veterinary Medicine, Trakia University, 6000, Stara Zagora, Bulgaria. 2. Department of Clinical Science, University of Bergen, 5020, Bergen, Norway. 3. Department of Internal Diseases, Faculty of Veterinary Medicine, Trakia University, 6000, Stara Zagora, Bulgaria. 4. Aker BioMarine Antarctic AS, Fjordalléen 16, 0115, Oslo, Norway. 5. Molecular Biology, Immunology and Medical Genetics, Medical Faculty, Trakia University, 6000, Stara Zagora, Bulgaria. 6. Department of Veterinary Anatomy, Histology and Embryology, Faculty of Veterinary Medicine, Trakia University, 6000, Stara Zagora, Bulgaria. 7. Department of Veterinary Microbiology, Infection and Parasitic Diseases, Faculty of Veterinary Medicine, Trakia University, 6000, Stara Zagora, Bulgaria. 8. Department of Pharmacology, Animal Physiology and Physiological Chemistry, Faculty of Veterinary Medicine, Trakia University, 6000, Stara Zagora, Bulgaria. iv_p63@yahoo.com.
Abstract
PURPOSE: This study was conducted to investigate the effect of fish oil (FO) and krill oil (KO) supplementation on glucose tolerance in obese New Zealand white rabbits. METHODS: The experiments were carried out with 24 male rabbits randomly divided into four groups: KO-castrated, treated with KO; FO-castrated, treated with FO; C-castrated, non-treated; NC-non-castrated, non-treated. At the end of treatment period (2 months), an intravenous glucose tolerance test (IVGTT) was performed in all rabbits. RESULTS: Fasting blood glucose concentrations in FO and KO animals were significantly lower than in group C. The blood glucose concentrations in FO- and KO-treated animals returned to initial values after 30 and 60 min of IVGTT, respectively. In liver, carnitine palmitoyltransferase 2 (Cpt2) and 3-hydroxy-3-methyl-glutaryl-CoA synthase 2 (Hmgcs2) genes were significantly increased in FO-fed rabbits compared with the C group. Acetyl-CoA carboxylase alpha (Acaca) expression was significantly reduced in both KO- and FO-fed rabbits. In skeletal muscle, Hmgcs2 and Cd36 were significantly higher in KO-fed rabbits compared with the C group. Acaca expression was significantly lower in KO- and FO-fed rabbits compared with the C group. CONCLUSION: The present results indicate that FO and KO supplementation decreases fasting blood glucose and improves glucose tolerance in obese New Zealand white rabbits. This could be ascribed to the ameliorated insulin sensitivity and insulin secretion and modified gene expressions of some key enzymes involved in β-oxidation and lipogenesis in liver and skeletal muscle.
PURPOSE: This study was conducted to investigate the effect of fish oil (FO) and krill oil (KO) supplementation on glucose tolerance in obese New Zealand white rabbits. METHODS: The experiments were carried out with 24 male rabbits randomly divided into four groups: KO-castrated, treated with KO; FO-castrated, treated with FO; C-castrated, non-treated; NC-non-castrated, non-treated. At the end of treatment period (2 months), an intravenous glucose tolerance test (IVGTT) was performed in all rabbits. RESULTS: Fasting blood glucose concentrations in FO and KO animals were significantly lower than in group C. The blood glucose concentrations in FO- and KO-treated animals returned to initial values after 30 and 60 min of IVGTT, respectively. In liver, carnitine palmitoyltransferase 2 (Cpt2) and 3-hydroxy-3-methyl-glutaryl-CoA synthase 2 (Hmgcs2) genes were significantly increased in FO-fed rabbits compared with the C group. Acetyl-CoA carboxylase alpha (Acaca) expression was significantly reduced in both KO- and FO-fed rabbits. In skeletal muscle, Hmgcs2 and Cd36 were significantly higher in KO-fed rabbits compared with the C group. Acaca expression was significantly lower in KO- and FO-fed rabbits compared with the C group. CONCLUSION: The present results indicate that FO and KO supplementation decreases fasting blood glucose and improves glucose tolerance in obese New Zealand white rabbits. This could be ascribed to the ameliorated insulin sensitivity and insulin secretion and modified gene expressions of some key enzymes involved in β-oxidation and lipogenesis in liver and skeletal muscle.
Authors: K Ajiro; M Sawamura; K Ikeda; Y Nara; M Nishimura; H Ishida; Y Seino; Y Yamori Journal: Clin Exp Pharmacol Physiol Date: 2000 May-Jun Impact factor: 2.557
Authors: S Kitajima; M Morimoto; E Liu; T Koike; Y Higaki; Y Taura; K Mamba; K Itamoto; T Watanabe; K Tsutsumi; N Yamada; J Fan Journal: Diabetologia Date: 2004-06-25 Impact factor: 10.122