| Literature DB >> 25306352 |
Haroon Khalid Syed1, Kai Bin Liew2, Gabriel Onn Kit Loh3, Kok Khiang Peh4.
Abstract
A stability-indicating HPLC-UV method for the determination of curcumin in Curcuma longa extract and emulsion was developed. The system suitability parameters, theoretical plates (N), tailing factor (T), capacity factor (K'), height equivalent of a theoretical plate (H) and resolution (Rs) were calculated. Stress degradation studies (acid, base, oxidation, heat and UV light) of curcumin were performed in emulsion. It was found that N>6500, T<1.1, K' was 2.68-3.75, HETP about 37 and Rs was 1.8. The method was linear from 2 to 200 μg/mL with a correlation coefficient of 0.9998. The intra-day precision and accuracy for curcumin were ⩽0.87% and ⩽2.0%, while the inter-day precision and accuracy values were ⩽2.1% and ⩽-1.92. Curcumin degraded in emulsion under acid, alkali and UV light. In conclusion, the stability-indicating method could be employed to determine curcumin in bulk and emulsions.Entities:
Keywords: Curcumin; Stability indicating HPLC–UV method; Stress degradation study; System suitability
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Year: 2014 PMID： 25306352 DOI： 10.1016/j.foodchem.2014.08.066
Source DB: PubMed Journal: Food Chem ISSN： 0308-8146 Impact factor: 7.514