| Literature DB >> 25306246 |
Nutan Prasain1, Man Ryul Lee2, Sasidhar Vemula1, Jonathan Luke Meador1, Momoko Yoshimoto1, Michael J Ferkowicz1, Alexa Fett1, Manav Gupta1, Brian M Rapp3, Mohammad Reza Saadatzadeh3, Michael Ginsberg4, Olivier Elemento5, Younghee Lee6, Sherry L Voytik-Harbin7, Hyung Min Chung8, Ki Sung Hong9, Emma Reid10, Christina L O'Neill10, Reinhold J Medina10, Alan W Stitt10, Michael P Murphy3, Shahin Rafii4, Hal E Broxmeyer2, Mervin C Yoder1.
Abstract
The ability to differentiate human pluripotent stem cells into endothelial cells with properties of cord-blood endothelial colony-forming cells (CB-ECFCs) may enable the derivation of clinically relevant numbers of highly proliferative blood vessel-forming cells to restore endothelial function in patients with vascular disease. We describe a protocol to convert human induced pluripotent stem cells (hiPSCs) or embryonic stem cells (hESCs) into cells similar to CB-ECFCs at an efficiency of >10(8) ECFCs produced from each starting pluripotent stem cell. The CB-ECFC-like cells display a stable endothelial phenotype with high clonal proliferative potential and the capacity to form human vessels in mice and to repair the ischemic mouse retina and limb, and they lack teratoma formation potential. We identify Neuropilin-1 (NRP-1)-mediated activation of KDR signaling through VEGF165 as a critical mechanism for the emergence and maintenance of CB-ECFC-like cells.Entities:
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Year: 2014 PMID： 25306246 PMCID： PMC4318247 DOI： 10.1038/nbt.3048
Source DB: PubMed Journal: Nat Biotechnol ISSN： 1087-0156 Impact factor: 54.908