| Literature DB >> 25305740 |
Franziska Janusch1, Gesine Scherz2, Siegrun A I Mohring1, Gerd Hamscher3.
Abstract
The application of antibiotics including fluoroquinolones to farming animals is widespread and may lead to the development of antibiotic resistance and other environmental effects. To calculate environmental loads and for a proper risk assessment it is necessary to determine the antibiotic concentration in feces. Therefore, a new liquid-liquid extraction method combined with HPLC-MS/MS for the detection of marbofloxacin, ciprofloxacin, enrofloxacin and difloxacin in chicken feces was developed. Recoveries ranged from 51.0% to 83.5%. LOQs were between 0.10 and 1.09μg/kg. Feces of chickens treated with an enrofloxacin dosage of 10mg/kg bodyweight revealed maximum enrofloxacin and ciprofloxacin concentrations of 61.3 and 18.8mg/kg. Both antibiotics could be detected in feces up to two days after the last application in notable amounts (∼1mg/kg). Thus, feces of recently medicated chickens should not be used as a fertilizer without any further processing.Entities:
Keywords: Chicken; Feces; Fluoroquinolones; LC–MS/MS; Liquid–liquid extraction
Mesh：
Substances：
Year: 2014 PMID： 25305740 DOI： 10.1016/j.etap.2014.09.011
Source DB: PubMed Journal: Environ Toxicol Pharmacol ISSN： 1382-6689 Impact factor: 4.860