N Deepak Kumar1, Rama S Kalluru2, Shafie Ahmed3, A Abhilashini4, Thumu Jayaprakash5, Roopadevi Garlapati6, Butti Sowmya7, K Narasimha Reddy8. 1. Senior Lecturer, Department of Conservative Dentistry and Endodontics, RVS College of Dental Sciences , Coimbatore, Tamil Nadu, India . 2. Senior Lecturer, Department of Conservative Dentistry and Endodontics, Sibar Institute of Dental Sciences , Guntur, Andhra Pradesh, India . 3. Professor, Department of Conservative Dentistry and Endodontics, Raja Muthaiah Dental College , Chidambaram, Tamil Nadu, India . 4. Senior Lecturer, Department of Pedodontics, RVS College of Dental Sciences , Coimbatore, Tamil Nadu, India . 5. Professor, Department of Conservative Dentistry and Endodontics, St.Joseph Dental College , Eluru, Andhra Pradesh, India . 6. Senior Lecturer, Department of Conservative Dentistry and Endodontics, Kamineni Institute of Dental Sciences , Narketpally, Andhra Pradesh, India . 7. Lecturer, Department of Pedodontics, at Sibar Institute of Dental Sciences , Guntur, Andhra Pradesh, India . 8. Reader, Department of Conservative Dentistry and Endodontics, Sibar Institute of Dental Sciences , Guntur, India .
Abstract
AIM: To compare the antibacterial efficacy of Manuka honey against E.faecalis and E.coli. MATERIALS AND METHODS: Escherichia coli (ATCC-25922) and Enterococcus faecalis (ATCC-29212) were separately inoculated in the nutrient broth and incubated at 37(o)C for 24-48 hrs. Bacterial samples were kept in contact with each disinfectant solution for varying intervals of time. Once the test time had elapsed 10μL of the bacterial dilutions were plated on Mueller-Hinton agar and incubated for 24-48 hrs at 37°C to estimate the density. Study of the disinfection process with respect to time and Modeling was done. RESULTS: The mean value of the antimicrobial activity of Manuka honey against E.coli and E.faecalis are 1.55 and 0.36 respectively and are relatively higher. It shows that there is a significant difference among the various root canal disinfectant groups against E. coli and E. feacalis. (p<0.001) CONCLUSION: Manuka honey is shown to be a potential root canal disinfectant against gram positive and gram negative bacterial pathogens.
AIM: To compare the antibacterial efficacy of Manuka honey against E.faecalis and E.coli. MATERIALS AND METHODS:Escherichia coli (ATCC-25922) and Enterococcus faecalis (ATCC-29212) were separately inoculated in the nutrient broth and incubated at 37(o)C for 24-48 hrs. Bacterial samples were kept in contact with each disinfectant solution for varying intervals of time. Once the test time had elapsed 10μL of the bacterial dilutions were plated on Mueller-Hinton agar and incubated for 24-48 hrs at 37°C to estimate the density. Study of the disinfection process with respect to time and Modeling was done. RESULTS: The mean value of the antimicrobial activity of Manuka honey against E.coli and E.faecalis are 1.55 and 0.36 respectively and are relatively higher. It shows that there is a significant difference among the various root canal disinfectant groups against E. coli and E. feacalis. (p<0.001) CONCLUSION:Manuka honey is shown to be a potential root canal disinfectant against gram positive and gram negative bacterial pathogens.
Entities:
Keywords:
Antibacterial efficacy; Death kinetics; Manuka honey