Roopadevi Garlapati1, Bhuvan Shome Venigalla2, Jayaprada Reddy Surakanti3, Jayaprakash Thumu4, Krishna Chaitanya Chennamaneni5, Rama S Kalluru6. 1. Senior Lecturer, Department of Conservative Dentistry and Endodontics, Kamineni Institute of Dental Sciences , Narketpally, Telangana, India . 2. Professor and Head of Department, Department of Conservative Dentistry and Endodontics, Sri Sai College of Dental Surgery , Vikarabad, Telangana, India . 3. Professor, Department of Conservative Dentistry and Endodontics, Kamineni Institute of Dental Sciences , Narketpally, Telangana, India . 4. Professor and Head of Department, Department of Conservative Dentistry and Endodontics, St. Joseph Dental College , Eluru, Andhra Pradesh, India . 5. Reader, Department of Conservative Dentistry and Endodontics, Sri Sai College of Dental Surgery , Vikarabad, Telangana, India . 6. Senior Lecturer, Department of Conservative Dentistry and Endodontics, Sibar Institute of Dental Sciences , Andhra Pradesh, India .
Abstract
INTRODUCTION: One of the main goals of endodontic treatment is root canal disinfection and to prevent subsequent chances of reinfection. Adjuvant to instrumentation, root canal irrigants are required to eliminate the bacteria found on the root canal walls and lateral canals within the dentinal tubules. AIM: To measure and compare the antibacterial efficacy of two antibiotics as experimental root canal irrigating solutions against Enterococcus faecalis (E. faecalis). MATERIALS AND METHODS: Fifteen Brain Heart Infusion agar plates were inoculated with Enterococcus faecalis-American Type Culture Collection (ATCC) 29212. 5 micrograms (mcg) Sparfloxacin discs, 30mcg Augmentin discs, and sterile paper test discs saturated with 2% Chlorhexidine (CHX), 3% Sodium Hypochlorite (NaOCl) and 5% NaOCl solutions were placed on agar plates. Sodium Chloride 0.9% (NaCl) paper discs were used as controls. Fifteen plates were incubated aerobically at 37°C. Results were expressed as per the terms of the diameter of the inhibition zone. RESULTS: Results suggested a statistically significant difference in the zones of inhibition between five irrigating solutions (p < 0.001). CONCLUSION: Although, zones of inhibition were found in all the groups, 5mcg Sparfloxacin and 30mcg Augmentin showed maximum antimicrobial activity against E.faecalis.
INTRODUCTION: One of the main goals of endodontic treatment is root canal disinfection and to prevent subsequent chances of reinfection. Adjuvant to instrumentation, root canal irrigants are required to eliminate the bacteria found on the root canal walls and lateral canals within the dentinal tubules. AIM: To measure and compare the antibacterial efficacy of two antibiotics as experimental root canal irrigating solutions against Enterococcus faecalis (E. faecalis). MATERIALS AND METHODS: Fifteen Brain Heart Infusion agar plates were inoculated with Enterococcus faecalis-American Type Culture Collection (ATCC) 29212. 5 micrograms (mcg) Sparfloxacin discs, 30mcg Augmentin discs, and sterile paper test discs saturated with 2% Chlorhexidine (CHX), 3% Sodium Hypochlorite (NaOCl) and 5% NaOCl solutions were placed on agar plates. Sodium Chloride 0.9% (NaCl) paper discs were used as controls. Fifteen plates were incubated aerobically at 37°C. Results were expressed as per the terms of the diameter of the inhibition zone. RESULTS: Results suggested a statistically significant difference in the zones of inhibition between five irrigating solutions (p < 0.001). CONCLUSION: Although, zones of inhibition were found in all the groups, 5mcg Sparfloxacin and 30mcg Augmentin showed maximum antimicrobial activity against E.faecalis.
Authors: M A Cohen; M D Huband; G B Mailloux; S L Yoder; G E Roland; C L Heifetz Journal: Diagn Microbiol Infect Dis Date: 1991 Sep-Oct Impact factor: 2.803