Literature DB >> 25293958

Multiple paramagnetic effects through a tagged reporter protein.

Aldo R Camacho-Zarco1, Francesca Munari, Melanie Wegstroth, Wei-Min Liu, Marcellus Ubbink, Stefan Becker, Markus Zweckstetter.   

Abstract

Paramagnetic effects provide unique information about the structure and dynamics of biomolecules. We developed a method in which the lanthanoid tag is not directly attached to the protein of interest, but instead to a "reporter" protein, which binds and then transmits paramagnetic information to the target. The designed method allows access to a large number of paramagnetic restraints and residual dipolar couplings produced from independent molecular alignments in high-molecular-weight proteins with unknown 3D structure.
© 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Entities:  

Keywords:  NMR spectroscopy; lanthanoids; paramagnetism; proteins; structural biology

Mesh：

Substances：

Year:  2014        PMID： 25293958      PMCID： PMC5650053          DOI： 10.1002/anie.201408615

Source DB:  PubMed          Journal:  Angew Chem Int Ed Engl        ISSN： 1433-7851            Impact factor:   15.336


  37 in total

1.  A solution model of the complex formed by adrenodoxin and adrenodoxin reductase determined by paramagnetic NMR spectroscopy.

Authors:  Peter H J Keizers; Berna Mersinli; Wolfgang Reinle; Julia Donauer; Yoshitaka Hiruma; Frank Hannemann; Mark Overhand; Rita Bernhardt; Marcellus Ubbink
Journal:  Biochemistry       Date:  2010-08-17       Impact factor: 3.162

2.  Engineering encodable lanthanide-binding tags into loop regions of proteins.

Authors:  Katja Barthelmes; Anne M Reynolds; Ezra Peisach; Hendrik R A Jonker; Nicholas J DeNunzio; Karen N Allen; Barbara Imperiali; Harald Schwalbe
Journal:  J Am Chem Soc       Date:  2011-02-02       Impact factor: 15.419

3.  Numbat: an interactive software tool for fitting Deltachi-tensors to molecular coordinates using pseudocontact shifts.

Authors:  Christophe Schmitz; Mitchell J Stanton-Cook; Xun-Cheng Su; Gottfried Otting; Thomas Huber
Journal:  J Biomol NMR       Date:  2008-06-24       Impact factor: 2.835

4.  DOTA-M8: An extremely rigid, high-affinity lanthanide chelating tag for PCS NMR spectroscopy.

Authors:  Daniel Häussinger; Jie-rong Huang; Stephan Grzesiek
Journal:  J Am Chem Soc       Date:  2009-10-21       Impact factor: 15.419

5.  Use of relaxation enhancements in a paramagnetic environment for the structure determination of proteins using NMR spectroscopy.

Authors:  Tobias Madl; Wolfgang Bermel; Klaus Zangger
Journal:  Angew Chem Int Ed Engl       Date:  2009       Impact factor: 15.336

6.  A pH-sensitive, colorful, lanthanide-chelating paramagnetic NMR probe.

Authors:  Wei-Min Liu; Peter H J Keizers; Mathias A S Hass; Anneloes Blok; Monika Timmer; Alexi J C Sarris; Mark Overhand; Marcellus Ubbink
Journal:  J Am Chem Soc       Date:  2012-10-05       Impact factor: 15.419

7.  Tuning the affinity for lanthanides of calcium binding proteins.

Authors:  Ivano Bertini; Ioannis Gelis; Nikolaos Katsaros; Claudio Luchinat; Alessandro Provenzani
Journal:  Biochemistry       Date:  2003-07-08       Impact factor: 3.162

8.  Design, synthesis, and evaluation of a lanthanide chelating protein probe: CLaNP-5 yields predictable paramagnetic effects independent of environment.

Authors:  Peter H J Keizers; Athanasios Saragliadis; Yoshitaka Hiruma; Mark Overhand; Marcellus Ubbink
Journal:  J Am Chem Soc       Date:  2008-10-01       Impact factor: 15.419

9.  Double-lanthanide-binding tags: design, photophysical properties, and NMR applications.

Authors:  Langdon J Martin; Martin J Hähnke; Mark Nitz; Jens Wöhnert; Nicholas R Silvaggi; Karen N Allen; Harald Schwalbe; Barbara Imperiali
Journal:  J Am Chem Soc       Date:  2007-05-12       Impact factor: 15.419

10.  Improved accuracy of 15N-1H scalar and residual dipolar couplings from gradient-enhanced IPAP-HSQC experiments on protonated proteins.

Authors:  Lishan Yao; Jinfa Ying; Ad Bax
Journal:  J Biomol NMR       Date:  2009-02-10       Impact factor: 2.835
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  6 in total

1.  Identification of primary and secondary UBA footprints on the surface of ubiquitin in cell-mimicking crowded solution.

Authors:  Francesca Munari; Andrea Bortot; Serena Zanzoni; Mariapina D'Onofrio; David Fushman; Michael Assfalg
Journal:  FEBS Lett       Date:  2017-03-19       Impact factor: 4.124

2.  A minor conformation of a lanthanide tag on adenylate kinase characterized by paramagnetic relaxation dispersion NMR spectroscopy.

Authors:  Mathias A S Hass; Wei-Min Liu; Roman V Agafonov; Renee Otten; Lien A Phung; Jesika T Schilder; Dorothee Kern; Marcellus Ubbink
Journal:  J Biomol NMR       Date:  2015-01-08       Impact factor: 2.835

Review 3.  Computational modeling of RNA 3D structure based on experimental data.

Authors:  Almudena Ponce-Salvatierra; Katarzyna Merdas; Chandran Nithin; Pritha Ghosh; Sunandan Mukherjee; Janusz M Bujnicki
Journal:  Biosci Rep       Date:  2019-02-08       Impact factor: 3.840

4.  Paramagnetic Ligand Tagging To Identify Protein Binding Sites.

Authors:  Ulrika Brath; Shashikala I Swamy; Alberte X Veiga; Ching-Chieh Tung; Filip Van Petegem; Máté Erdélyi
Journal:  J Am Chem Soc       Date:  2015-08-27       Impact factor: 15.419

Review 5.  "Rules of Engagement" of Protein-Glycoconjugate Interactions: A Molecular View Achievable by using NMR Spectroscopy and Molecular Modeling.

Authors:  Roberta Marchetti; Serge Perez; Ana Arda; Anne Imberty; Jesus Jimenez-Barbero; Alba Silipo; Antonio Molinaro
Journal:  ChemistryOpen       Date:  2016-06-07       Impact factor: 2.911

6.  A Two-Armed Probe for In-Cell DEER Measurements on Proteins*.

Authors:  Qing Miao; Enrico Zurlo; Donny de Bruin; Joeri A J Wondergem; Monika Timmer; Anneloes Blok; Doris Heinrich; Mark Overhand; Martina Huber; Marcellus Ubbink
Journal:  Chemistry       Date:  2020-11-17       Impact factor: 5.236
  6 in total

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