| Literature DB >> 25287212 |
Abstract
Subcellular, sequence-specific detection of RNA in vivo is a powerful tool to study the macromolecular transport that occurs through plasmodesmata. The RNA-binding domain of Pumilio proteins can be engineered to bind RNA sequences of choice and fused to fluorescent proteins for RNA imaging. This chapter describes the construction of a Pumilio-based imaging system to track the RNA of Tobacco mosaic virus in vivo, and practical aspects of RNA live-cell imaging.Entities:
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Year: 2015 PMID： 25287212 DOI： 10.1007/978-1-4939-1523-1_20
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745