Ting-Feng Wu1, Chien-Feng Li2, Lan-Hsiang Chien3, Kun-Hung Shen4, Hsuan-Ying Huang5, Chia-Cheng Su3, Alex C Liao6. 1. Department of Biotechnology, Southern Taiwan University of Science and Technology, Tainan, Taiwan, Republic of China. 2. Department of Biotechnology, Southern Taiwan University of Science and Technology, Tainan, Taiwan, Republic of China; Department of Pathology, Chi-Mei Medical Center, Tainan, Taiwan, Republic of China; National Institute of Cancer Research, National Health Research Institutes, Tainan, Taiwan, Republic of China. 3. Department of Urology, Chi-Mei Medical Center, Tainan, Taiwan, Republic of China. 4. Department of Urology, Chi-Mei Medical Center, Tainan, Taiwan, Republic of China; Department of Optometry, Chung Hwa University of Medical Technology, Tainan, Taiwan, Republic of China; Department of Urology, Taipei Medical University, Taipei, Taiwan, Republic of China. Electronic address: robert.shen@msa.hinet.net. 5. Department of Pathology, Chang Gung Memorial Hospital-Kaohsiung Medical Center Chang Gung University College of Medicine, Kaohsiung, Taiwan, Republic of China. 6. Department of Urology, Chi-Mei Medical Center, Tainan, Taiwan, Republic of China; Department of Senior Citizen Service Management, Chia Nan University of Pharmacy and Science, Tainan, Taiwan, Republic of China.
Abstract
PURPOSE: Galectin-1 is highly expressed in various tumors and participates in various oncogenic processes. Our previous proteomics investigation demonstrated that galectin-1 is up-regulated in high compared to nonhigh grade lesions. Thus, in the current cohort study we clarified the correlation of galectin-1 over expression with various clinicopathological features and prognosis. MATERIALS AND METHODS: We selected 185 cases of consecutively treated primary localized bladder urothelial carcinoma for study. Transurethral resection of bladder tumor was performed in all patients followed by radical cystectomy in those with T2 to T4 tumors. Pathological slides were examined to determine cytoplasmic galectin-1 immuno-expression and correlate galectin-1 dysregulation with various clinicopathological factors and disease specific survival. RESULTS: Positive galectin-1 immuno-expression in tumors was significantly linked to pT status (p = 0.0295), histological grade (p = 0.037), vascular invasion (p = 0.0287) and nodal status (p = 0.0012). Galectin-1 over expression in tumors significantly predicted disease specific survival at the univariate (p = 0.0002) and multivariate levels (p = 0.03, HR 2.438, 95% CI 1.090-5.451). In situ hybridization indicated that the LGALS1 gene was amplified in 43 specimens in an independent cohort of 56 snap frozen tumor specimens. Association analysis showed that an increased LGALS1 mRNA level was linked to bladder urothelial carcinoma invasiveness (p = 0.016) and LGALS1 gene amplification was significantly associated the amount of GAL-1 protein in tumors (p <0.0001). On the univariate level gene amplification was also closely linked to disease specific survival (p = 0.0006). CONCLUSIONS: These results reveal that galectin-1 over expression is a possible independent factor for bladder cancer prognosis.
PURPOSE:Galectin-1 is highly expressed in various tumors and participates in various oncogenic processes. Our previous proteomics investigation demonstrated that galectin-1 is up-regulated in high compared to nonhigh grade lesions. Thus, in the current cohort study we clarified the correlation of galectin-1 over expression with various clinicopathological features and prognosis. MATERIALS AND METHODS: We selected 185 cases of consecutively treated primary localized bladder urothelial carcinoma for study. Transurethral resection of bladder tumor was performed in all patients followed by radical cystectomy in those with T2 to T4 tumors. Pathological slides were examined to determine cytoplasmic galectin-1 immuno-expression and correlate galectin-1 dysregulation with various clinicopathological factors and disease specific survival. RESULTS: Positive galectin-1 immuno-expression in tumors was significantly linked to pT status (p = 0.0295), histological grade (p = 0.037), vascular invasion (p = 0.0287) and nodal status (p = 0.0012). Galectin-1 over expression in tumors significantly predicted disease specific survival at the univariate (p = 0.0002) and multivariate levels (p = 0.03, HR 2.438, 95% CI 1.090-5.451). In situ hybridization indicated that the LGALS1 gene was amplified in 43 specimens in an independent cohort of 56 snap frozen tumor specimens. Association analysis showed that an increased LGALS1 mRNA level was linked to bladder urothelial carcinoma invasiveness (p = 0.016) and LGALS1 gene amplification was significantly associated the amount of GAL-1protein in tumors (p <0.0001). On the univariate level gene amplification was also closely linked to disease specific survival (p = 0.0006). CONCLUSIONS: These results reveal that galectin-1 over expression is a possible independent factor for bladder cancer prognosis.
Authors: Patricia M R Pereira; Sheryl Roberts; Flávio Figueira; João P C Tomé; Thomas Reiner; Jason S Lewis Journal: J Nucl Med Date: 2020-01-31 Impact factor: 10.057
Authors: Pallavi Sethi; Amar Jyoti; Elden P Swindell; Ryan Chan; Ulrich W Langner; Jonathan M Feddock; Radhakrishnan Nagarajan; Thomas V O'Halloran; Meenakshi Upreti Journal: Nanomedicine Date: 2015-08-15 Impact factor: 5.307