| Literature DB >> 25280994 |
Soichiro Funaki1, Toshinobu Nakamura2, Tsunetoshi Nakatani3, Hiroki Umehara4, Hiroyuki Nakashima4, Toru Nakano5.
Abstract
DNA methylation is a key epigenetic regulator in mammals, and the dynamic balance between methylation and demethylation impacts various processes, from development to disease. DNA methylation is erased during replication when DNA methyltransferase 1 (DNMT1) fails to methylate the daughter strand, in a process known as passive DNA demethylation. We found that the enforced expression of Stella (also known as PGC7, Dppa3), a maternal factor required for the maintenance of DNA methylation in early embryos, induced global DNA demethylation in NIH3T3 cells. This demethylation was caused by the binding of Stella to Np95 (also known as Uhrf1, ICBP90) and the subsequent inhibition of DNMT1 recruitment. Considering that impaired DNA methylation profiles are associated with various developmental or disease phenomena, Stella may be a powerful tool with which to study the biological effects of global DNA hypomethylation.Entities:
Keywords: DNA demethylation; Dnmt1; Np95; Stella
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Year: 2014 PMID： 25280994 DOI： 10.1016/j.bbrc.2014.09.101
Source DB: PubMed Journal: Biochem Biophys Res Commun ISSN： 0006-291X Impact factor: 3.575