Literature DB >> 25277746

A multiplexed droplet digital PCR assay performs better than qPCR on inhibition prone samples.

Ruth Hall Sedlak1, Jane Kuypers1, Keith R Jerome2.   

Abstract

We demonstrate the development of a multiplex droplet digital PCR assay for human cytomegalovirus (CMV), human adenovirus species F, and an internal plasmid control that may be useful for PCR inhibition-prone clinical samples. This assay performs better on inhibition-prone stool samples than a quantitative PCR assay for CMV and is the first published clinical virology droplet digital PCR assay to incorporate an internal control.
Copyright © 2014 Elsevier Inc. All rights reserved.

Entities:  

Keywords:  Digital PCR; Inhibition; Quantitative PCR; Stool

Mesh：

Year:  2014        PMID： 25277746     DOI： 10.1016/j.diagmicrobio.2014.09.004

Source DB:  PubMed          Journal:  Diagn Microbiol Infect Dis        ISSN： 0732-8893            Impact factor:   2.803


  25 in total

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