Study of the N-glycoprotein biosynthesis through dolichol intermediates in the mitochondrial membranes.

1. In the mitochondria, the biosynthesis of N-glycoprotein products, through the dolichol intermediates pathway, appears in the outer and in the inner membranes. 2. The biosynthesis of dolichol-pyrophosphoryl-N-acetyl-glucosamine, dolichol-pyrophosphoryl-di-N-acetylchitobiose, dolichol-phosphoryl-glucose and dolichol-phosphoryl-mannose is effective in both membranes. 3. The lipid-linked oligosaccharides biosynthesized in both membranes contain high mannose-type oligosaccharides ranging in size from Man9-GlcNac2 to Man4-GlcNac2. 4. The assembly of the dolichol-pyrophosphoryl-oligosaccharides on the trimannosidic core begins by the elongation of the alpha-1,3 mannose branch in the outer membrane and of the alpha-1,6 mannose branch in the inner membrane.