Literature DB >> 2525902

Functions of the COOH-terminal region of cyclodextrin glucanotransferase of alkalophilic Bacillus sp. #1011: relation to catalyzing activity and pH stability.

K Kimura1, S Kataoka, A Nakamura, T Takano, S Kobayashi, K Yamane.   

Abstract

Cyclodextrin glucanotransferase (beta-CGTase) of alkalophilic Bacillus sp. #1011 degrades starch to mainly beta-cyclodextrin (beta-CD). This enzyme is considered to contain an extra-polypeptide in its COOH-terminal region in addition to its NH2-terminal domain which exhibits the starch-degrading activity. To analyze the functions of this extra-polypeptide in the beta-CGTase, two mutated enzymes, in which DNA regions encoding 10 or 13 amino acids from the COOH-terminus were deleted, were obtained. The mutated enzymes degraded starch to glucose, maltooligosaccharides and alpha-CD, in addition to beta-CD. Furthermore, the pH stability of the mutated enzymes in the alkaline pH range (pH 9-11) was reduced.

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Year:  1989        PMID： 2525902     DOI： 10.1016/0006-291x(89)91380-6

Source DB:  PubMed          Journal:  Biochem Biophys Res Commun        ISSN： 0006-291X            Impact factor:   3.575


  7 in total

1.  Characterization of an archaeal cyclodextrin glucanotransferase with a novel C-terminal domain.

Authors:  Naeem Rashid; Joel Cornista; Satoshi Ezaki; Toshiaki Fukui; Haruyuki Atomi; Tadayuki Imanaka
Journal:  J Bacteriol       Date:  2002-02       Impact factor: 3.490

2.  Characteristics of two forms of alpha-amylases and structural implication.

Authors:  K Ohdan; T Kuriki; H Kaneko; J Shimada; T Takada; Z Fujimoto; H Mizuno; S Okada
Journal:  Appl Environ Microbiol       Date:  1999-10       Impact factor: 4.792

3.  Modes of action of acarbose hydrolysis and transglycosylation catalyzed by a thermostable maltogenic amylase, the gene for which was cloned from a Thermus strain.

Authors:  T J Kim; M J Kim; B C Kim; J C Kim; T K Cheong; J W Kim; K H Park
Journal:  Appl Environ Microbiol       Date:  1999-04       Impact factor: 4.792

4.  Cyclization characteristics of cyclodextrin glucanotransferase are conferred by the NH2-terminal region of the enzyme.

Authors:  S Fujiwara; H Kakihara; K B Woo; A Lejeune; M Kanemoto; K Sakaguchi; T Imanaka
Journal:  Appl Environ Microbiol       Date:  1992-12       Impact factor: 4.792

5.  Cloning and sequence analysis of the cyclomaltodextrinase gene from Bacillus sphaericus and expression in Escherichia coli cells.

Authors:  T Oguma; A Matsuyama; M Kikuchi; E Nakano
Journal:  Appl Microbiol Biotechnol       Date:  1993-05       Impact factor: 4.813

6.  Analysis of mutations in cyclodextrin glucanotransferase from Bacillus stearothermophilus which affect cyclization characteristics and thermostability.

Authors:  S Fujiwara; H Kakihara; K Sakaguchi; T Imanaka
Journal:  J Bacteriol       Date:  1992-11       Impact factor: 3.490

7.  Aspergillus Oryzae S2 α-Amylase Domain C Involvement in Activity and Specificity: In Vivo Proteolysis, Molecular and Docking Studies.

Authors:  Mouna Sahnoun; Sonia Jemli; Sahar Trabelsi; Leila Ayadi; Samir Bejar
Journal:  PLoS One       Date:  2016-04-21       Impact factor: 3.240
  7 in total

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