Literature DB >> 25255882

Stable small quantum dots for synaptic receptor tracking on live neurons.

En Cai1, Pinghua Ge, Sang Hak Lee, Okunola Jeyifous, Yong Wang, Yanxin Liu, Katie M Wilson, Sung Jun Lim, Michelle A Baird, John E Stone, Kwan Young Lee, Michael W Davidson, Hee Jung Chung, Klaus Schulten, Andrew M Smith, William N Green, Paul R Selvin.   

Abstract

We developed a coating method to produce functionalized small quantum dots (sQDs), about 9 nm in diameter, that were stable for over a month. We made sQDs in four emission wavelengths, from 527 to 655 nm and with different functional groups. AMPA receptors on live neurons were labeled with sQDs and postsynaptic density proteins were visualized with super-resolution microscopy. Their diffusion behavior indicates that sQDs access the synaptic clefts significantly more often than commercial QDs.
© 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Entities:  

Keywords:  AMPA receptors; fluorescent probes; quantum dots; single-molecule studies

Mesh：

Substances：

Year:  2014        PMID： 25255882      PMCID： PMC4240739          DOI： 10.1002/anie.201405735

Source DB:  PubMed          Journal:  Angew Chem Int Ed Engl        ISSN： 1433-7851            Impact factor:   15.336


  34 in total

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Journal:  Nat Neurosci       Date:  2004-06-20       Impact factor: 24.884

Review 2.  Quantum dots for live cells, in vivo imaging, and diagnostics.

Authors:  X Michalet; F F Pinaud; L A Bentolila; J M Tsay; S Doose; J J Li; G Sundaresan; A M Wu; S S Gambhir; S Weiss
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4.  Determination of absolute protein numbers in single synapses by a GFP-based calibration technique.

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Journal:  Nat Methods       Date:  2005-09       Impact factor: 28.547

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Review 6.  Cloned glutamate receptors.

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Journal:  Annu Rev Neurosci       Date:  1994       Impact factor: 12.449

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9.  Formation of targeted monovalent quantum dots by steric exclusion.

Authors:  Justin Farlow; Daeha Seo; Kyle E Broaders; Marcus J Taylor; Zev J Gartner; Young-Wook Jun
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  25 in total

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2.  Covalent Protein Labeling and Improved Single-Molecule Optical Properties of Aqueous CdSe/CdS Quantum Dots.

Authors:  Sara M Wichner; Victor R Mann; Alexander S Powers; Maya A Segal; Mustafa Mir; Jigar N Bandaria; Mark A DeWitt; Xavier Darzacq; Ahmet Yildiz; Bruce E Cohen
Journal:  ACS Nano       Date:  2017-06-21       Impact factor: 15.881

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7.  Quantum Dots for Improved Single-Molecule Localization Microscopy.

Authors:  Jennifer M Urban; Wesley Chiang; Jennetta W Hammond; Nicole M B Cogan; Angela Litzburg; Rebeckah Burke; Harry A Stern; Harris A Gelbard; Bradley L Nilsson; Todd D Krauss
Journal:  J Phys Chem B       Date:  2021-03-08       Impact factor: 2.991

8.  Bright sub-20-nm cathodoluminescent nanoprobes for electron microscopy.

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10.  Ligand-conjugated quantum dots for fast sub-diffraction protein tracking in acute brain slices.

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