Satoko Hirobe-Jahn1, Simone Harsch2, Olga Renner3, Dominique Richter4, Oliver Müller5, Eduard F Stange6. 1. Dr. Margarete Fischer-Bosch Institute for Clinical Pharmacology and University of Tuebingen, Stuttgart, Germany. Electronic address: S_HirobeJahn@uni-hohenheim.de. 2. Dr. Margarete Fischer-Bosch Institute for Clinical Pharmacology and University of Tuebingen, Stuttgart, Germany. Electronic address: Simone.Harsch@ikp-stuttgart.de. 3. Dr. Margarete Fischer-Bosch Institute for Clinical Pharmacology and University of Tuebingen, Stuttgart, Germany. Electronic address: Olga.Renner@ikp-stuttgart.de. 4. Dr. Margarete Fischer-Bosch Institute for Clinical Pharmacology and University of Tuebingen, Stuttgart, Germany. Electronic address: Dominique.Richter@ikp-stuttgart.de. 5. Department of Internal Medicine I, Robert Bosch Hospital, Stuttgart, Germany. Electronic address: Oliver.Mueller@rbk.de. 6. Department of Internal Medicine I, Robert Bosch Hospital, Stuttgart, Germany. Electronic address: Eduard.Stange@rbk.de.
Abstract
BACKGROUND AND AIM: Impairment of bile acid homeostasis is the most important risk factor of gallstone disease. Thereby the bile acid sensor farnesoid X receptor (FXR) plays a pivotal role in hepatic and intestinal bile acid metabolism. In this explorative study, the FXR gene was investigated to identify gene variants, associated with gallstone formation in a Caucasian population. METHODS: Sequencing of the FXR gene was conducted in a randomly selected cohort of gallstone carriers (n=30) and control subjects (n=16) from Stuttgart, Germany. Genomic DNA was obtained from blood leukocytes. Genotype frequencies were established in the total cohort (controls: n=133, gallstone carriers: n=74). For expression analysis, total RNA and protein were isolated from ileal biopsies. RESULTS: The sequencing showed the sole appearance of 10 SNPs in gallstone carriers. Further genotype analysis revealed significant gender- and weight-dependent frequency differences of 3 SNPs between gallstone carriers and controls in males (rs35724: OR=4.73, P=0.022) and normal weight subjects (rs11110385: OR=3.67, P=0.027; rs11110386: OR=3.67, P=0.027) applying the 11+12<>22 allele model. Furthermore, rs11110385 carriers showed a significantly decreased FXR protein expression (11+12<>22: P=0.003). Significant mRNA expression differences between lean rs11110385 carriers and non-carriers were observed in FXR target genes (decrease: ILBP: P=0.042, OSTalpha: P=0.071, FGF19: P=0.011. Increase: LRH1: P=0.044). CONCLUSIONS: Three FXR gene variants (rs35724, rs11110385, rs11110386) were identified as potential susceptibility factors for cholelithiasis in a German cohort in gender- and weight-dependent manners. Thereby the tag SNP rs11110385 seemed to influence the activation of the FXR gene.
BACKGROUND AND AIM: Impairment of bile acid homeostasis is the most important risk factor of gallstone disease. Thereby the bile acid sensor farnesoid X receptor (FXR) plays a pivotal role in hepatic and intestinal bile acid metabolism. In this explorative study, the FXR gene was investigated to identify gene variants, associated with gallstone formation in a Caucasian population. METHODS: Sequencing of the FXR gene was conducted in a randomly selected cohort of gallstone carriers (n=30) and control subjects (n=16) from Stuttgart, Germany. Genomic DNA was obtained from blood leukocytes. Genotype frequencies were established in the total cohort (controls: n=133, gallstone carriers: n=74). For expression analysis, total RNA and protein were isolated from ileal biopsies. RESULTS: The sequencing showed the sole appearance of 10 SNPs in gallstone carriers. Further genotype analysis revealed significant gender- and weight-dependent frequency differences of 3 SNPs between gallstone carriers and controls in males (rs35724: OR=4.73, P=0.022) and normal weight subjects (rs11110385: OR=3.67, P=0.027; rs11110386: OR=3.67, P=0.027) applying the 11+12<>22 allele model. Furthermore, rs11110385 carriers showed a significantly decreased FXR protein expression (11+12<>22: P=0.003). Significant mRNA expression differences between lean rs11110385 carriers and non-carriers were observed in FXR target genes (decrease: ILBP: P=0.042, OSTalpha: P=0.071, FGF19: P=0.011. Increase: LRH1: P=0.044). CONCLUSIONS: Three FXR gene variants (rs35724, rs11110385, rs11110386) were identified as potential susceptibility factors for cholelithiasis in a German cohort in gender- and weight-dependent manners. Thereby the tag SNP rs11110385 seemed to influence the activation of the FXR gene.
Authors: Georg Semmler; Benedikt Simbrunner; Bernhard Scheiner; Philipp Schwabl; Rafael Paternostro; Theresa Bucsics; Albert Friedrich Stättermayer; David Bauer; Matthias Pinter; Peter Ferenci; Michael Trauner; Mattias Mandorfer; Thomas Reiberger Journal: J Gastroenterol Hepatol Date: 2019-06-14 Impact factor: 4.029
Authors: Richard N Appleby; Jonathan D Nolan; Ian M Johnston; Sanjeev S Pattni; Jessica Fox; Julian Rf Walters Journal: BMJ Open Gastroenterol Date: 2017-10-26