| Literature DB >> 25241167 |
Ranran Cheng1, Jin Peng2, Yonghong Yan3, Peili Cao3, Jiewei Wang4, Chen Qiu3, Lichun Tang3, Di Liu3, Li Tang3, Jianping Jin5, Xingxu Huang6, Fuchu He7, Pumin Zhang8.
Abstract
We developed an adenovirus-based CRISPR/Cas9 system for gene editing in vivo. In the liver, we demonstrated that the system could reach the level of tissue-specific gene knockout, resulting in phenotypic changes. Given the wide spectrum of cell types susceptible to adenoviral infection, and the fact that adenoviral genome rarely integrates into its host cell genome, we believe the adenovirus-based CRISPR/Cas9 system will find applications in a variety of experimental settings.Entities:
Keywords: Adenoviruses; CRISPR/Cas9; Gene editing; Liver
Mesh：
Year: 2014 PMID： 25241167 DOI： 10.1016/j.febslet.2014.09.008
Source DB: PubMed Journal: FEBS Lett ISSN： 0014-5793 Impact factor: 4.124