| Literature DB >> 25232641 |
Romiza R Mazid1, Upulie Divisekera, Wenjuan Yang, Vijayaraghavan Ranganathan, Douglas R MacFarlane, Christina Cortez-Jugo, Wenlong Cheng.
Abstract
We report substantially enhanced biological stability of siRNA in a hydrated ionic liquid (IL) based on buffered choline dihydrogen phosphate (CDP). The results show that hydrated CDP IL substantially prolongs the shelf-life of siRNA for up to three months in the presence of RNase A. Remarkably, siRNA stored in the IL remained active and eGFP siRNA exhibited clear gene knockdown effects in eGFP-expressing HeLa cells.Entities:
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Year: 2014 PMID： 25232641 DOI： 10.1039/c4cc05086j
Source DB: PubMed Journal: Chem Commun (Camb) ISSN： 1359-7345 Impact factor: 6.222