Literature DB >> 25232168

Evaluation of high-resolution melting curve analysis of ligation-mediated real-time PCR, a rapid method for epidemiological typing of ESKAPE (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter Species) pathogens.

Hanna Woksepp1, Anna Ryberg2, Hanna Billström3, Anita Hällgren4, Lennart E Nilsson4, Britt-Inger Marklund5, Barbro Olsson-Liljequist3, Thomas Schön6.   

Abstract

A single-tube method, ligation-mediated real-time PCR high-resolution melt analysis (LMqPCR HRMA), was modified for the rapid typing of Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter spp. (ESKAPE) pathogens. A 97% agreement (60/62 isolates) was achieved in comparison to pulsed-field gel electrophoresis (PFGE) results, which indicates that LMqPCR HRMA is a rapid and accurate screening tool for monitoring nosocomial outbreaks.
Copyright © 2014, American Society for Microbiology. All Rights Reserved.

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Year:  2014        PMID： 25232168      PMCID： PMC4313309          DOI： 10.1128/JCM.02537-14

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  14 in total

1.  Ligation mediated PCR performed at low denaturation temperatures--PCR melting profiles.

Authors:  Aleksander Masny; Andrzej Płucienniczak
Journal:  Nucleic Acids Res       Date:  2003-09-15       Impact factor: 16.971

Review 2.  Rapid whole-genome sequencing of bacterial pathogens in the clinical microbiology laboratory--pipe dream or reality?

Authors:  M E Török; S J Peacock
Journal:  J Antimicrob Chemother       Date:  2012-06-22       Impact factor: 5.790

3.  High-resolution melting-curve analysis of ligation-mediated real-time PCR for rapid evaluation of an epidemiological outbreak of extended-spectrum-beta-lactamase-producing Escherichia coli.

Authors:  Hanna Woksepp; Cecilia Jernberg; Maria Tärnberg; Anna Ryberg; Alma Brolund; Michaela Nordvall; Barbro Olsson-Liljequist; Karin Tegmark Wisell; Hans-Jürg Monstein; Lennart E Nilsson; Thomas Schön
Journal:  J Clin Microbiol       Date:  2011-09-28       Impact factor: 5.948

Review 4.  Overview of molecular typing methods for outbreak detection and epidemiological surveillance.

Authors:  A J Sabat; A Budimir; D Nashev; R Sá-Leão; J m van Dijl; F Laurent; H Grundmann; A W Friedrich
Journal:  Euro Surveill       Date:  2013-01-24

Review 5.  DNA fingerprinting of medically important microorganisms by use of PCR.

Authors:  A van Belkum
Journal:  Clin Microbiol Rev       Date:  1994-04       Impact factor: 26.132

6.  Guidelines for the validation and application of typing methods for use in bacterial epidemiology.

Authors:  A van Belkum; P T Tassios; L Dijkshoorn; S Haeggman; B Cookson; N K Fry; V Fussing; J Green; E Feil; P Gerner-Smidt; S Brisse; M Struelens
Journal:  Clin Microbiol Infect       Date:  2007-10       Impact factor: 8.067

7.  The future of antibiotics and resistance.

Authors:  Brad Spellberg; John G Bartlett; David N Gilbert
Journal:  N Engl J Med       Date:  2013-01-24       Impact factor: 91.245

8.  Rapid bacterial whole-genome sequencing to enhance diagnostic and public health microbiology.

Authors:  Sandra Reuter; Matthew J Ellington; Edward J P Cartwright; Claudio U Köser; M Estée Török; Theodore Gouliouris; Simon R Harris; Nicholas M Brown; Matthew T G Holden; Mike Quail; Julian Parkhill; Geoffrey P Smith; Stephen D Bentley; Sharon J Peacock
Journal:  JAMA Intern Med       Date:  2013-08-12       Impact factor: 21.873

Review 9.  Routine use of microbial whole genome sequencing in diagnostic and public health microbiology.

Authors:  Claudio U Köser; Matthew J Ellington; Edward J P Cartwright; Stephen H Gillespie; Nicholas M Brown; Mark Farrington; Matthew T G Holden; Gordon Dougan; Stephen D Bentley; Julian Parkhill; Sharon J Peacock
Journal:  PLoS Pathog       Date:  2012-08-02       Impact factor: 6.823

Review 10.  Whole-genome sequencing in bacteriology: state of the art.

Authors:  Michael J Dark
Journal:  Infect Drug Resist       Date:  2013-10-08       Impact factor: 4.003
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  7 in total

1.  Third-generation-cephalosporin-resistant Klebsiella pneumoniae isolates from humans and companion animals in Switzerland: spread of a DHA-producing sequence type 11 clone in a veterinary setting.

Authors:  Nadia Wohlwend; Andrea Endimiani; Thierry Francey; Vincent Perreten
Journal:  Antimicrob Agents Chemother       Date:  2015-03-02       Impact factor: 5.191

2.  Multilocus Sequence Typing Analysis of Carbapenem-Resistant Acinetobacter baumannii in a Chinese Burns Institute.

Authors:  Guangtao Huang; Supeng Yin; Yali Gong; Xia Zhao; Lingyun Zou; Bei Jiang; Zhiwei Dong; Yu Chen; Jing Chen; Shouguang Jin; Zhiqiang Yuan; Yizhi Peng
Journal:  Front Microbiol       Date:  2016-11-09       Impact factor: 5.640

3.  High-Resolution Melting Analysis for Rapid Detection of Sequence Type 131 Escherichia coli.

Authors:  Lucas B Harrison; Nancy D Hanson
Journal:  Antimicrob Agents Chemother       Date:  2017-05-24       Impact factor: 5.191

4.  Antimicrobial Resistance, Virulence Genes, and Biofilm Formation Capacity Among Enterococcus species From Yaks in Aba Tibetan Autonomous Prefecture, China.

Authors:  Pengfei Cui; Lan Feng; Lan Zhang; Juan He; Tianwu An; Xue Fu; Cui Li; Xiaodong Zhao; Yaru Zhai; Hao Li; Wenjun Yan; Huade Li; Xiaolin Luo; Changwei Lei; Hongning Wang; Xin Yang
Journal:  Front Microbiol       Date:  2020-06-12       Impact factor: 5.640

Review 5.  A systematic review and meta-analysis on the prevalence of vancomycin-resistant enterococci (VRE) among Nigerians.

Authors:  Oluwatosin Qawiyy Orababa; Jeffry Difiye Soriwei; Samuel Oluwamayowa Akinsuyi; Utibeima Udo Essiet; Olusola Michael Solesi
Journal:  Porto Biomed J       Date:  2021-02-11

6.  Optimization and development of high-resolution melting curve analysis (HRMA) assay for detection of New Delhi metallo-β-lactamase (NDM) producing Pseudomonas aeruginosa.

Authors:  Sanaz Dehbashi; Hamed Tahmasebi; Mohammad Yousef Alikhani; Fariba Keramat; Mohammad Reza Arabestani
Journal:  AIMS Microbiol       Date:  2022-05-09

7.  Development of high-resolution melting curve analysis in rapid detection of vanA gene, Enterococcus faecalis, and Enterococcus faecium from clinical isolates.

Authors:  Sanaz Dehbashi; Hamed Tahmasebi; Parinaz Sedighi; Faeze Davarian; Mohammad Reza Arabestani
Journal:  Trop Med Health       Date:  2020-02-18
  7 in total

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