Saban Gürcan1, Melek Tikveşli1, Sedat Ustündağ2, Beyza Ener3. 1. Department of Medical Microbiology, Trakya University Faculty of Medicine, Edirne, Turkey. 2. Department of Nephrology, Trakya University Faculty of Medicine, Edirne, Turkey. 3. Department of Medical Microbiology, Uludağ University Faculty of Medicine, Bursa, Turkey.
Abstract
BACKGROUND: Aspergillus lentulus was described as a new species in 2005 but it was isolated from Turkey for the first time. CASE REPORT: A. lentulus was isolated as the cause of pneumonia from a patient who had renal transplantation 4 months ago. The patient received immunosuppressive treatment after transplantation. A. lentulus was isolated from his sputum as an agent in pneumonia developed 4 months after the transplantation. Leukocytes, blastospores, and hyphae were seen in both Gram- and Giemsastained smears of the sputum. The isolate was identified by using the Maren A. Klich algorithm and molecular methods and confirmed by the reference laboratory of the CBS Fungal Biodiversity Centre (The Netherlands). In the susceptibility tests of the isolate, minimal inhibitory concentrations for amphotericin B, voriconazole, posaconazole, and caspofungin were found to be 0.5 μg/mL, 0.25 μg/mL, 0.125 μg/mL, and 0.25 μg/mL, respectively. The patient recovered with voriconazole treatment (2×200 mg/day). CONCLUSION: The use of the molecular tests is important for identification of A. lentulus strains because they are very easily confused with A.fumigatus strains according to phenotypic characteristics.
BACKGROUND:Aspergillus lentulus was described as a new species in 2005 but it was isolated from Turkey for the first time. CASE REPORT: A. lentulus was isolated as the cause of pneumonia from a patient who had renal transplantation 4 months ago. The patient received immunosuppressive treatment after transplantation. A. lentulus was isolated from his sputum as an agent in pneumonia developed 4 months after the transplantation. Leukocytes, blastospores, and hyphae were seen in both Gram- and Giemsastained smears of the sputum. The isolate was identified by using the Maren A. Klich algorithm and molecular methods and confirmed by the reference laboratory of the CBS Fungal Biodiversity Centre (The Netherlands). In the susceptibility tests of the isolate, minimal inhibitory concentrations for amphotericin B, voriconazole, posaconazole, and caspofungin were found to be 0.5 μg/mL, 0.25 μg/mL, 0.125 μg/mL, and 0.25 μg/mL, respectively. The patient recovered with voriconazole treatment (2×200 mg/day). CONCLUSION: The use of the molecular tests is important for identification of A. lentulus strains because they are very easily confused with A.fumigatus strains according to phenotypic characteristics.
Authors: Emilia Mellado; Laura Alcazar-Fuoli; Manuel Cuenca-Estrella; Juan L Rodriguez-Tudela Journal: Antimicrob Agents Chemother Date: 2011-09-26 Impact factor: 5.191
Authors: Laura Alcazar-Fuoli; Isabel Cuesta; Juan L Rodriguez-Tudela; Manuel Cuenca-Estrella; Dominique Sanglard; Emilia Mellado Journal: Int J Antimicrob Agents Date: 2011-08-25 Impact factor: 5.283
Authors: K L Mortensen; H K Johansen; K Fuursted; J D Knudsen; B Gahrn-Hansen; R H Jensen; S J Howard; M C Arendrup Journal: Eur J Clin Microbiol Infect Dis Date: 2011-05-04 Impact factor: 3.267
Authors: A Zbinden; A Imhof; M J Wilhelm; F Ruschitzka; P Wild; G V Bloemberg; N J Mueller Journal: Transpl Infect Dis Date: 2012-09-19 Impact factor: 2.228
Authors: Graciela Montenegro; Silvia Sánchez Puch; Virginia M Jewtuchowicz; Maria V Pinoni; Silvia Relloso; Elena Temporitti; Cristina A Iovannitti; Maria T Mujica Journal: J Med Microbiol Date: 2009-03 Impact factor: 2.472
Authors: Allison R McMullen; Meghan A Wallace; David H Pincus; Kathy Wilkey; C A Burnham Journal: J Clin Microbiol Date: 2016-05-25 Impact factor: 5.948