| Literature DB >> 25194849 |
Martin Soste1, Rita Hrabakova2, Stefanie Wanka3, Andre Melnik1, Paul Boersema1, Alessio Maiolica4, Timon Wernas1, Marco Tognetti1, Christian von Mering3, Paola Picotti1.
Abstract
We describe a proteomic screening approach based on the concept of sentinel proteins, biological markers whose change in abundance characterizes the activation state of a given cellular process. Our sentinel assay simultaneously probed 188 biological processes in Saccharomyces cerevisiae exposed to a set of environmental perturbations. The approach can be applied to analyze responses to large sets of uncharacterized perturbations in high throughput.Entities:
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Year: 2014 PMID: 25194849 DOI: 10.1038/nmeth.3101
Source DB: PubMed Journal: Nat Methods ISSN: 1548-7091 Impact factor: 28.547