Literature DB >> 25194849

A sentinel protein assay for simultaneously quantifying cellular processes.

Martin Soste1, Rita Hrabakova2, Stefanie Wanka3, Andre Melnik1, Paul Boersema1, Alessio Maiolica4, Timon Wernas1, Marco Tognetti1, Christian von Mering3, Paola Picotti1.   

Abstract

We describe a proteomic screening approach based on the concept of sentinel proteins, biological markers whose change in abundance characterizes the activation state of a given cellular process. Our sentinel assay simultaneously probed 188 biological processes in Saccharomyces cerevisiae exposed to a set of environmental perturbations. The approach can be applied to analyze responses to large sets of uncharacterized perturbations in high throughput.

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Year:  2014        PMID: 25194849     DOI: 10.1038/nmeth.3101

Source DB:  PubMed          Journal:  Nat Methods        ISSN: 1548-7091            Impact factor:   28.547


  51 in total

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Journal:  Genetics       Date:  2011-12-29       Impact factor: 4.562

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6.  Full dynamic range proteome analysis of S. cerevisiae by targeted proteomics.

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7.  Cooperative binding of heat shock factor to the yeast HSP82 promoter in vivo and in vitro.

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Journal:  Mol Cell Biol       Date:  1999-03       Impact factor: 4.272

8.  Hexokinase 2 from Saccharomyces cerevisiae: regulation of oligomeric structure by in vivo phosphorylation at serine-14.

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9.  Secreted 3-isopropylmalate methyl ester signals invasive growth during amino acid starvation in Saccharomyces cerevisiae.

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Journal:  Biochemistry       Date:  2007-12-20       Impact factor: 3.162

10.  Using iRT, a normalized retention time for more targeted measurement of peptides.

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  28 in total

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4.  Reduced-representation Phosphosignatures Measured by Quantitative Targeted MS Capture Cellular States and Enable Large-scale Comparison of Drug-induced Phenotypes.

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Review 8.  Single-cell proteomic analysis.

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Review 9.  Application of targeted mass spectrometry in bottom-up proteomics for systems biology research.

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Journal:  J Proteomics       Date:  2018-02-13       Impact factor: 4.044

Review 10.  The Library of Integrated Network-Based Cellular Signatures NIH Program: System-Level Cataloging of Human Cells Response to Perturbations.

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Journal:  Cell Syst       Date:  2017-11-29       Impact factor: 10.304

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