Literature DB >> 25194233

To pool or not to pool? Impact of the use of individual and pooled fecal samples for in vitro fermentation studies.

Marisol Aguirre1, Javier Ramiro-Garcia2, Marjorie E Koenen3, Koen Venema4.   

Abstract

This study investigated the stability and the activity of the microbiota from a single and a pool of donors in the TNO in vitro model of the colon (TIM-2 system). Our findings demonstrate the suitability of the preparation of a pool of fecal sample to be used for fermentation experiments.
Copyright © 2014 Elsevier B.V. All rights reserved.

Keywords:  Fecal inoculum preparation; Fermentation; Gut microbiota; In vitro model; Pooling

Mesh:

Substances:

Year:  2014        PMID: 25194233     DOI: 10.1016/j.mimet.2014.08.022

Source DB:  PubMed          Journal:  J Microbiol Methods        ISSN: 0167-7012            Impact factor:   2.363


  19 in total

1.  Comparative methods for fecal sample storage to preserve gut microbial structure and function in an in vitro model of the human colon.

Authors:  Charlotte Deschamps; Elora Fournier; Ophélie Uriot; Frédérique Lajoie; Cécile Verdier; Sophie Comtet-Marre; Muriel Thomas; Nathalie Kapel; Claire Cherbuy; Monique Alric; Mathieu Almeida; Lucie Etienne-Mesmin; Stéphanie Blanquet-Diot
Journal:  Appl Microbiol Biotechnol       Date:  2020-10-21       Impact factor: 4.813

2.  Human Adult Microbiota in a Static Colon Model: AhR Transcriptional Activity at the Crossroads of Host-Microbe Interaction.

Authors:  Elizabeth Goya-Jorge; Irma Gonza; Pauline Bondue; Caroline Douny; Bernard Taminiau; Georges Daube; Marie-Louise Scippo; Véronique Delcenserie
Journal:  Foods       Date:  2022-06-30

3.  Impact of a fermented soy beverage supplemented with acerola by-product on the gut microbiota from lean and obese subjects using an in vitro model of the human colon.

Authors:  Antonio Diogo Silva Vieira; Carlota Bussolo de Souza; Marina Padilha; Erwin Gerard Zoetendal; Hauke Smidt; Susana Marta Isay Saad; Koen Venema
Journal:  Appl Microbiol Biotechnol       Date:  2021-05-03       Impact factor: 4.813

4.  In vitro characterization of the impact of different substrates on metabolite production, energy extraction and composition of gut microbiota from lean and obese subjects.

Authors:  Marisol Aguirre; Daisy M A E Jonkers; Freddy J Troost; Guus Roeselers; Koen Venema
Journal:  PLoS One       Date:  2014-11-26       Impact factor: 3.240

5.  Microbial Metabolism Shifts Towards an Adverse Profile with Supplementary Iron in the TIM-2 In vitro Model of the Human Colon.

Authors:  Guus A M Kortman; Bas E Dutilh; Annet J H Maathuis; Udo F Engelke; Jos Boekhorst; Kevin P Keegan; Fiona G G Nielsen; Jason Betley; Jacqueline C Weir; Zoya Kingsbury; Leo A J Kluijtmans; Dorine W Swinkels; Koen Venema; Harold Tjalsma
Journal:  Front Microbiol       Date:  2016-01-06       Impact factor: 5.640

6.  The Gut Microbiota from Lean and Obese Subjects Contribute Differently to the Fermentation of Arabinogalactan and Inulin.

Authors:  Marisol Aguirre; Carlota Bussolo de Souza; Koen Venema
Journal:  PLoS One       Date:  2016-07-13       Impact factor: 3.240

7.  Determination of Resistant Starch Assimilating Bacteria in Fecal Samples of Mice by In vitro RNA-Based Stable Isotope Probing.

Authors:  Elena Herrmann; Wayne Young; Douglas Rosendale; Ralf Conrad; Christian U Riedel; Markus Egert
Journal:  Front Microbiol       Date:  2017-07-24       Impact factor: 5.640

8.  Divergent short-chain fatty acid production and succession of colonic microbiota arise in fermentation of variously-sized wheat bran fractions.

Authors:  Yunus E Tuncil; Riya D Thakkar; Arianna D Romero Marcia; Bruce R Hamaker; Stephen R Lindemann
Journal:  Sci Rep       Date:  2018-11-09       Impact factor: 4.379

9.  Bifidobacterium breve UCC2003 Exopolysaccharide Modulates the Early Life Microbiota by Acting as a Potential Dietary Substrate.

Authors:  Deborah Püngel; Agatha Treveil; Matthew J Dalby; Shabhonam Caim; Ian J Colquhoun; Catherine Booth; Jennifer Ketskemety; Tamas Korcsmaros; Douwe van Sinderen; Melissa Ae Lawson; Lindsay J Hall
Journal:  Nutrients       Date:  2020-03-29       Impact factor: 5.717

10.  In Vitro Fermentation Behavior of Isomalto/Malto-Polysaccharides Using Human Fecal Inoculum Indicates Prebiotic Potential.

Authors:  Fangjie Gu; Klaudyna Borewicz; Bernadette Richter; Pieter H van der Zaal; Hauke Smidt; Pieter L Buwalda; Henk A Schols
Journal:  Mol Nutr Food Res       Date:  2018-05-28       Impact factor: 5.914

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