Fouzia Sultana1, Hafsa Afroz2, Afroz Jahan1, Md Fakruddin3, Suvamoy Datta2. 1. Department of Microbiology, Primeasia University, Banani, Dhaka-1213, Bangladesh ; Department of Microbiology, Institute of Public Health (IPH), Dhaka-1212, Bangladesh. 2. Department of Microbiology, Primeasia University, Banani, Dhaka-1213, Bangladesh. 3. Industrial Microbiology Laboratory, IFST, BCSIR, Dhaka-1205, Bangladesh.
Abstract
OBJECTIVE: To investigate the bacterial load and antibiotic resistance pattern of bacterial isolates obtained from (ready to cook) frozen food samples of animal origin in Dhaka, Bangladesh. METHODS: A total of 20 samples of frozen ready to cook food of animal origin were purchased from different separate grocery stores in Dhaka, Bangladesh. Bacteria were isolated and identified based on the basis of biochemical properties. RESULTS: A total of 57 isolates has been isolated from 20 samples, of them 35.08% were Gram positive and 64.92% were Gram negative organisms. Highest percentages of isolated organisms were Staphylococcocus spp. (24.56%), Alcaligene spp. (17.54%), Klebshiella spp. (12.28%) and the lowest percentages of organisms were Enterococcus spp., Actinobacillus spp. and Proteus spp. Antibiogram results clearly showed that levofloxacin and imipenem were the most effective drug against the isolates. The less effective antibiotics were chloramphenicol and nalidixic acid and resistance was highest against ciprofloxacin. The most contaminated food was chicken nuggets. CONCLUSIONS: This type of frozen food contaminated with multi-antibiotic resistant microorganisms can be potential vehicles for transmitting food-borne diseases.
OBJECTIVE: To investigate the bacterial load and antibiotic resistance pattern of bacterial isolates obtained from (ready to cook) frozen food samples of animal origin in Dhaka, Bangladesh. METHODS: A total of 20 samples of frozen ready to cook food of animal origin were purchased from different separate grocery stores in Dhaka, Bangladesh. Bacteria were isolated and identified based on the basis of biochemical properties. RESULTS: A total of 57 isolates has been isolated from 20 samples, of them 35.08% were Gram positive and 64.92% were Gram negative organisms. Highest percentages of isolated organisms were Staphylococcocus spp. (24.56%), Alcaligene spp. (17.54%), Klebshiella spp. (12.28%) and the lowest percentages of organisms were Enterococcus spp., Actinobacillus spp. and Proteus spp. Antibiogram results clearly showed that levofloxacin and imipenem were the most effective drug against the isolates. The less effective antibiotics were chloramphenicol and nalidixic acid and resistance was highest against ciprofloxacin. The most contaminated food was chicken nuggets. CONCLUSIONS: This type of frozen food contaminated with multi-antibiotic resistant microorganisms can be potential vehicles for transmitting food-borne diseases.
Authors: Ayman Elbehiry; Eman Marzouk; Musaad Aldubaib; Ihab Moussa; Adil Abalkhail; Mai Ibrahem; Mohamed Hamada; Wael Sindi; Feras Alzaben; Abdulaziz Mohammad Almuzaini; Abdelazeem M Algammal; Mohammed Rawway Journal: AMB Express Date: 2022-05-09 Impact factor: 4.126
Authors: Shahneaz Ali Khan; Mohammed Ashif Imtiaz; Md Abu Sayeed; Amir Hossan Shaikat; Mohammad Mahmudul Hassan Journal: BMC Vet Res Date: 2020-08-24 Impact factor: 2.741