Distribution of Kaiso protein in mouse tissues.

The Kaiso protein was originally described as a BTB/POZ zinc-finger transcription factor and a p120-catenin-binding partner. It is a DNA methylation-dependent transcriptional repressor, but its biological role in mice is still unknown. Here, we characterized a Kaiso-specific antibody by examining Kaiso protein distribution by immunofluorescence microscopy in the following tissues and cell types of adult mice: skin, small intestine, mammary glands, urinary bladder, and others. This study is the first to demonstrate that Kaiso is expressed in most of the examined tissues. Kaiso was localized to the nucleus in almost all tissues. However, it was primarily cytoplasmic in photoreceptor cells in the eye (rods and cones). Furthermore, Kaiso is expressed in a specific subset of male germ cells that are characterized by partly positive PLZF and Bmi-1 staining. In this study, we present the first confirmation of the reliability of expression data using Kaiso knockout mice.