Raphael Chukwuma Ekeanyanwu1, Obioma Uzoma Njoku2. 1. Department of Biochemistry, Faculty of Sciences, Imo State University Owerri, Imo State, Nigeria. 2. Department of Biochemistry, Faculty of Biological Sciences, University of Nigeria Nsukka, Enugu State, Nigeria.
Abstract
OBJECTIVE: To investigate the effects of the flavonoid rich fraction of Monodora tenuifolia seed on the haematology, histopathology and liver profile of Wistar albino rats. METHODS: Toxicity study was investigated on the flavonoid rich fraction of Monodora tenuifolia in rats administered different concentrations orally for 28 d using standard laboratory procedures. RESULTS: The LD50 of the flavonoid rich fraction was found to be above 5 000 mg/kg body weight in mice observed for 48 h. After the Day 14, biochemical markers of liver injury such as serum alanine aminotransferase, and aspartate aminotransferase decreased significantly (P<0.05 at doses of 100 and 200 mg/kg body weight and P<0.01 at 400 mg/kg) while serum alkaline phosphatase increased non-significantly (P>0.05). There was non-significant (P>0.05) effect observed across the groups in the levels of serum total protein, albumin, globulin, urea and creatinine. The result of histological examination showed various degrees of peribiliary hepatitis after the Day 14 which fizzled out after the Day 28. CONCLUSIONS: The result therefore suggests that the seed extract is potentially safe.
OBJECTIVE: To investigate the effects of the flavonoid rich fraction of Monodora tenuifolia seed on the haematology, histopathology and liver profile of Wistar albino rats. METHODS:Toxicity study was investigated on the flavonoid rich fraction of Monodora tenuifolia in rats administered different concentrations orally for 28 d using standard laboratory procedures. RESULTS: The LD50 of the flavonoid rich fraction was found to be above 5 000 mg/kg body weight in mice observed for 48 h. After the Day 14, biochemical markers of liver injury such as serum alanine aminotransferase, and aspartate aminotransferase decreased significantly (P<0.05 at doses of 100 and 200 mg/kg body weight and P<0.01 at 400 mg/kg) while serum alkaline phosphatase increased non-significantly (P>0.05). There was non-significant (P>0.05) effect observed across the groups in the levels of serum total protein, albumin, globulin, urea and creatinine. The result of histological examination showed various degrees of peribiliary hepatitis after the Day 14 which fizzled out after the Day 28. CONCLUSIONS: The result therefore suggests that the seed extract is potentially safe.
Authors: Shaymaa Fadhel Abbas Albaayit; Yusuf Abba; Rasedee Abdullah; Noorlidah Abdullah Journal: Evid Based Complement Alternat Med Date: 2014-12-24 Impact factor: 2.629
Authors: Alisson Macário de Oliveira; Matheus da Silva Mesquita; Gabriela Cavalcante da Silva; Edeltrudes de Oliveira Lima; Paloma Lys de Medeiros; Patrícia Maria Guedes Paiva; Ivone Antônia de Souza; Thiago Henrique Napoleão Journal: Evid Based Complement Alternat Med Date: 2015-07-12 Impact factor: 2.629