Issam Alshami1, Ahmed E Alharbi1. 1. Department of Medical Microbiology and Immunology, College of Medicine, Taibah University, Almadinah, Saudi Arabia.
Abstract
OBJECTIVE: To explore the prevention of recurrent candiduria using natural based approaches and to study the antimicrobial effect of Hibiscus sabdariffa (H. sabdariffa) extract and the biofilm forming capacity of Candida albicans strains in the present of the H. sabdariffa extract. METHODS: In this particular study, six strains of fluconazole resistant Candida albicans isolated from recurrent candiduria were used. The susceptibility of fungal isolates, time-kill curves and biofilm forming capacity in the present of the H. sabdariffa extract were determined. RESULTS: Various levels minimum inhibitory concentration of the extract were observed against all the isolates. Minimum inhibitory concentration values ranged from 0.5 to 2.0 mg/mL. Time-kill experiment demonstrated that the effect was fungistatic. The biofilm inhibition assay results showed that H. sabdariffa extract inhibited biofilm production of all the isolates. CONCLUSIONS: The results of the study support the potential effect of H. sabdariffa extract for preventing recurrent candiduria and emphasize the significance of the plant extract approach as a potential antifungal agent.
OBJECTIVE: To explore the prevention of recurrent candiduria using natural based approaches and to study the antimicrobial effect of Hibiscus sabdariffa (H. sabdariffa) extract and the biofilm forming capacity of Candida albicans strains in the present of the H. sabdariffa extract. METHODS: In this particular study, six strains of fluconazole resistant Candida albicans isolated from recurrent candiduria were used. The susceptibility of fungal isolates, time-kill curves and biofilm forming capacity in the present of the H. sabdariffa extract were determined. RESULTS: Various levels minimum inhibitory concentration of the extract were observed against all the isolates. Minimum inhibitory concentration values ranged from 0.5 to 2.0 mg/mL. Time-kill experiment demonstrated that the effect was fungistatic. The biofilm inhibition assay results showed that H. sabdariffa extract inhibited biofilm production of all the isolates. CONCLUSIONS: The results of the study support the potential effect of H. sabdariffa extract for preventing recurrent candiduria and emphasize the significance of the plant extract approach as a potential antifungal agent.
Authors: Marjorie Fullerton; Janak Khatiwada; Jacqueline U Johnson; Shurrita Davis; Leonard L Williams Journal: J Med Food Date: 2011-05-06 Impact factor: 2.786
Authors: Moussa S Diarra; Glenn Block; Heidi Rempel; B Dave Oomah; Judy Harrison; Jason McCallum; Simon Boulanger; Éric Brouillette; Mariza Gattuso; François Malouin Journal: BMC Complement Altern Med Date: 2013-04-27 Impact factor: 3.659