| Literature DB >> 25179006 |
Carla Coltharp1, Xinxing Yang1, Jie Xiao2.
Abstract
This review highlights the quantitative capabilities of single-molecule localization-based superresolution imaging methods. In addition to revealing fine structural details, the molecule coordinate lists generated by these methods provide the critical ability to quantify the number, clustering, and colocalization of molecules with 10-50 nm resolution. Here we describe typical workflows and precautions for quantitative analysis of single-molecule superresolution images. These guidelines include potential pitfalls and essential control experiments, allowing critical assessment and interpretation of superresolution images.Entities:
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Year: 2014 PMID: 25179006 PMCID: PMC4252250 DOI: 10.1016/j.sbi.2014.08.008
Source DB: PubMed Journal: Curr Opin Struct Biol ISSN: 0959-440X Impact factor: 6.809