Literature DB >> 25179006

Quantitative analysis of single-molecule superresolution images.

Carla Coltharp1, Xinxing Yang1, Jie Xiao2.   

Abstract

This review highlights the quantitative capabilities of single-molecule localization-based superresolution imaging methods. In addition to revealing fine structural details, the molecule coordinate lists generated by these methods provide the critical ability to quantify the number, clustering, and colocalization of molecules with 10-50 nm resolution. Here we describe typical workflows and precautions for quantitative analysis of single-molecule superresolution images. These guidelines include potential pitfalls and essential control experiments, allowing critical assessment and interpretation of superresolution images.
Copyright © 2014 Elsevier Ltd. All rights reserved.

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Year:  2014        PMID: 25179006      PMCID: PMC4252250          DOI: 10.1016/j.sbi.2014.08.008

Source DB:  PubMed          Journal:  Curr Opin Struct Biol        ISSN: 0959-440X            Impact factor:   6.809


  67 in total

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7.  Influence of FtsZ GTPase activity and concentration on nanoscale Z-ring structure in vivo revealed by three-dimensional Superresolution imaging.

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