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Literature DB >> 25169774

Frontal affinity chromatography: a unique research tool for biospecific interaction that promotes glycobiology.

Kenichi Kasai¹.
1. Teikyo University.

Abstract

Combination of bioaffinity and chromatography gave birth to affinity chromatography. A further combination with frontal analysis resulted in creation of frontal affinity chromatography (FAC). This new versatile research tool enabled detailed analysis of weak interactions that play essential roles in living systems, especially those between complex saccharides and saccharide-binding proteins. FAC now becomes the best method for the investigation of saccharide-binding proteins (lectins) from viewpoints of sensitivity, accuracy, and efficiency, and is contributing greatly to the development of glycobiology. It opened a door leading to deeper understanding of the significance of saccharide recognition in life. The theory is also concisely described.

Entities: Chemical Disease Gene Species

Mesh：

Substances：
Lectins
Polysaccharides

Year: 2014 PMID： 25169774 PMCID： PMC4237894 DOI： 10.2183/pjab.90.215

Source DB: PubMed Journal: Proc Jpn Acad Ser B Phys Biol Sci ISSN： 0386-2208 Impact factor: 3.493

58 in total

Review 1. Trypsin and affinity chromatography.

Authors: K Kasai
Journal: J Chromatogr Date: 1992-04-24

2. Evidence that Caenorhabditis elegans 32-kDa beta-galactoside-binding protein is homologous to vertebrate beta-galactoside-binding lectins. cDNA cloning and deduced amino acid sequence.

Authors: J Hirabayashi; M Satoh; K Kasai
Journal: J Biol Chem Date: 1992-08-05 Impact factor: 5.157

3. Purification and characterization of beta-galactoside-binding proteins from Caenorhabditis elegans.

Authors: J Hirabayashi; M Satoh; Y Ohyama; K Kasai
Journal: J Biochem Date: 1992-05 Impact factor: 3.387

4. Effect of amino acid substitution by sited-directed mutagenesis on the carbohydrate recognition and stability of human 14-kDa beta-galactoside-binding lectin.

Authors: J Hirabayashi; K Kasai
Journal: J Biol Chem Date: 1991-12-15 Impact factor: 5.157

5. Purification and molecular characterization of a novel 16-kDa galectin from the nematode Caenorhabditis elegans.

Authors: J Hirabayashi; T Ubukata; K Kasai
Journal: J Biol Chem Date: 1996-02-02 Impact factor: 5.157

Review 6. The family of metazoan metal-independent beta-galactoside-binding lectins: structure, function and molecular evolution.

Authors: J Hirabayashi; K Kasai
Journal: Glycobiology Date: 1993-08 Impact factor: 4.313

7. Structural basis of preferential binding of fucose-containing saccharide by the Caenorhabditis elegans galectin LEC-6.

Authors: Hisayoshi Makyio; Tomoharu Takeuchi; Mayumi Tamura; Kazusa Nishiyama; Hideyo Takahashi; Hideaki Natsugari; Yoichiro Arata; Ken-Ichi Kasai; Yusuke Yamada; Soichi Wakatsuki; Ryuichi Kato
Journal: Glycobiology Date: 2013-03-12 Impact factor: 4.313

8. Carbohydrate-binding specificities of five lectins that bind to O-Glycosyl-linked carbohydrate chains. Quantitative analysis by frontal-affinity chromatography.

Authors: S Sueyoshi; T Tsuji; T Osawa
Journal: Carbohydr Res Date: 1988-07-15 Impact factor: 2.104

9. Characterization of thyroxine-albumin binding using high-performance affinity chromatography. I. Interactions at the warfarin and indole sites of albumin.

Authors: B Loun; D S Hage
Journal: J Chromatogr Date: 1992-09-02

10. Further evidence by site-directed mutagenesis that conserved hydrophilic residues form a carbohydrate-binding site of human galectin-1.

Authors: J Hirabayashi; K Kasai
Journal: Glycoconj J Date: 1994-10 Impact factor: 2.916

4 in total