Douglas Roberto Monteiro1, Aline Satie Takamiya2, Leonardo Perina Feresin2, Luiz Fernando Gorup3, Emerson Rodrigues de Camargo3, Alberto Carlos Botazzo Delbem2, Mariana Henriques4, Debora Barros Barbosa5. 1. Department of Pediatric Dentistry and Public Health, Araçatuba Dental School, Univ Estadual Paulista (UNESP), 16015-050 Araçatuba, São Paulo, Brazil. Electronic address: douglasrmonteiro@hotmail.com. 2. Department of Pediatric Dentistry and Public Health, Araçatuba Dental School, Univ Estadual Paulista (UNESP), 16015-050 Araçatuba, São Paulo, Brazil. 3. Department of Chemistry, Federal University of São Carlos (UFSCar), 13565-905 São Carlos, São Paulo, Brazil. 4. Institute for Biotechnology and Bioengineering, Department of Biological Engineering, University of Minho, 4710-057 Braga, Portugal. 5. Department of Dental Materials and Prosthodontics, Araçatuba Dental School, Univ Estadual Paulista (UNESP), 16015-050 Araçatuba, São Paulo, Brazil.
Abstract
PURPOSE: The aim of this study was to investigate the susceptibility of Candida albicans and Candida glabrata biofilm development, in their intermediate and maturation stages, to the influence of silver nanoparticles (SN). METHODS: SN (5 nm) suspensions were synthesized via reduction of silver nitrate by a solution of sodium citrate. These suspensions were used to treat Candida biofilms for five hours, grown on acrylic surfaces for 24-h (intermediate stage) and 48-h (maturation stage), and their efficacy was determined by total biomass (using crystal violet staining) and colony forming units (CFUs) quantification. RESULTS: SN promoted significant reductions (p<0.05) in the total biomass and number of CFUs of Candida biofilms, ranging from 23% to 51.5% and 0.63 to 1.59-log10, respectively. Moreover, there were no significant differences in the total biofilm biomass (p>0.05), when the different stages of biofilm development (24 or 48h) were exposed to SN. Comparing the number of CFUs between 24- and 48-h biofilms treated with SN, a significant difference (p<0.05) was found only for the C. albicans 324LA/94 strain. CONCLUSIONS: In general, the intermediate and maturation stages of biofilm development do not interfere in the susceptibility of C. albicans and C. glabrata biofilms to SN. These findings are fundamental for the deployment of new therapies aimed at preventing denture stomatitis.
PURPOSE: The aim of this study was to investigate the susceptibility of Candida albicans and Candida glabrata biofilm development, in their intermediate and maturation stages, to the influence of silver nanoparticles (SN). METHODS:SN (5 nm) suspensions were synthesized via reduction of silver nitrate by a solution of sodium citrate. These suspensions were used to treat Candida biofilms for five hours, grown on acrylic surfaces for 24-h (intermediate stage) and 48-h (maturation stage), and their efficacy was determined by total biomass (using crystal violet staining) and colony forming units (CFUs) quantification. RESULTS:SN promoted significant reductions (p<0.05) in the total biomass and number of CFUs of Candida biofilms, ranging from 23% to 51.5% and 0.63 to 1.59-log10, respectively. Moreover, there were no significant differences in the total biofilm biomass (p>0.05), when the different stages of biofilm development (24 or 48h) were exposed to SN. Comparing the number of CFUs between 24- and 48-h biofilms treated with SN, a significant difference (p<0.05) was found only for the C. albicans 324LA/94 strain. CONCLUSIONS: In general, the intermediate and maturation stages of biofilm development do not interfere in the susceptibility of C. albicans and C. glabrata biofilms to SN. These findings are fundamental for the deployment of new therapies aimed at preventing denture stomatitis.
Authors: Humberto H Lara; Liliana Ixtepan-Turrent; Miguel Jose Yacaman; Jose Lopez-Ribot Journal: ACS Appl Mater Interfaces Date: 2020-01-16 Impact factor: 9.229
Authors: Humberto H Lara; Dulce G Romero-Urbina; Christopher Pierce; Jose L Lopez-Ribot; M Josefina Arellano-Jiménez; Miguel Jose-Yacaman Journal: J Nanobiotechnology Date: 2015-12-15 Impact factor: 10.435