Literature DB >> 25156274

Fabrication of cell-laden macroporous biodegradable hydrogels with tunable porosities and pore sizes.

Limin Wang1, Steven Lu, Johnny Lam, F Kurtis Kasper, Antonios G Mikos.   

Abstract

In this work, we investigated a cytocompatible particulate leaching method for the fabrication of cell-laden macroporous hydrogels. We used dehydrated and uncrosslinked gelatin microspheres as leachable porogens to create macroporous oligo(poly(ethylene glycol) fumarate) hydrogels. Varying gelatin content and size resulted in a wide range of porosities and pore sizes, respectively. Encapsulated mesenchymal stem cells (MSCs) exhibited high viability immediately following the fabrication process, and culture of cell-laden hydrogels revealed improved cell viability with increasing porosity. Additionally, the osteogenic potential of the encapsulated MSCs was evaluated over 16 days. Overall, this study presents a robust method for the preparation of cell-laden macroporous hydrogels with desired porosity and pore size for tissue engineering applications.

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Year:  2014        PMID： 25156274      PMCID： PMC4346546          DOI： 10.1089/ten.TEC.2014.0224

Source DB:  PubMed          Journal:  Tissue Eng Part C Methods        ISSN： 1937-3384            Impact factor:   3.056


  49 in total

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3.  Influence of macroporous protein scaffolds on bone tissue engineering from bone marrow stem cells.

Authors:  Hyeon Joo Kim; Ung-Jin Kim; Gordana Vunjak-Novakovic; Byoung-Hyun Min; David L Kaplan
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4.  In vitro evaluation of macroporous hydrogels to facilitate stem cell infiltration, growth, and mineralization.

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5.  The effect of mean pore size on cell attachment, proliferation and migration in collagen-glycosaminoglycan scaffolds for bone tissue engineering.

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Review 6.  Controlling the porosity and microarchitecture of hydrogels for tissue engineering.

Authors:  Nasim Annabi; Jason W Nichol; Xia Zhong; Chengdong Ji; Sandeep Koshy; Ali Khademhosseini; Fariba Dehghani
Journal:  Tissue Eng Part B Rev       Date:  2010-08       Impact factor: 6.389

7.  Macroporous hydrogels upregulate osteogenic signal expression and promote bone regeneration.

Authors:  Martha W Betz; Andrew B Yeatts; William J Richbourg; John F Caccamese; Domenick P Coletti; Erin E Falco; John P Fisher
Journal:  Biomacromolecules       Date:  2010-05-10       Impact factor: 6.988

8.  Fabrication of three-dimensional porous cell-laden hydrogel for tissue engineering.

Authors:  Chang Mo Hwang; Shilpa Sant; Mahdokht Masaeli; Nezamoddin N Kachouie; Behnam Zamanian; Sang-Hoon Lee; Ali Khademhosseini
Journal:  Biofabrication       Date:  2010-09-08       Impact factor: 9.954

9.  Three-dimensional porous biodegradable polymeric scaffolds fabricated with biodegradable hydrogel porogens.

Authors:  Jinku Kim; Michael J Yaszemski; Lichun Lu
Journal:  Tissue Eng Part C Methods       Date:  2009-12       Impact factor: 3.056

10.  A factorial analysis of the combined effects of hydrogel fabrication parameters on the in vitro swelling and degradation of oligo(poly(ethylene glycol) fumarate) hydrogels.

Authors:  Johnny Lam; Kyobum Kim; Steven Lu; Yasuhiko Tabata; David W Scott; Antonios G Mikos; F Kurtis Kasper
Journal:  J Biomed Mater Res A       Date:  2013-11-15       Impact factor: 4.396
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  6 in total

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Review 3.  Recent Progress in Developing Injectable Matrices for Enhancing Cell Delivery and Tissue Regeneration.

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4.  Evaluation of Gelatin Microparticles as Adherent-Substrates for Mesenchymal Stem Cells in a Hydrogel Composite.

Authors:  Steven Lu; Esther J Lee; Johnny Lam; Yasuhiko Tabata; Antonios G Mikos
Journal:  Ann Biomed Eng       Date:  2016-03-02       Impact factor: 3.934

5.  Cellularizing hydrogel-based scaffolds to repair bone tissue: How to create a physiologically relevant micro-environment?

Authors:  Mathieu Maisani; Daniele Pezzoli; Olivier Chassande; Diego Mantovani
Journal:  J Tissue Eng       Date:  2017-06-08       Impact factor: 7.813

Review 6.  Hydrogel: A Potential Material for Bone Tissue Engineering Repairing the Segmental Mandibular Defect.

Authors:  D S Abdullah Al Maruf; Yohaann Ali Ghosh; Hai Xin; Kai Cheng; Payal Mukherjee; Jeremy Micah Crook; Gordon George Wallace; Travis Jacob Klein; Jonathan Robert Clark
Journal:  Polymers (Basel)       Date:  2022-10-05       Impact factor: 4.967
  6 in total

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