Federico Y Fontana1, Daniel A Keir2, Cecilia Bellotti1, Gabriela F De Roia3, Juan M Murias4, Silvia Pogliaghi5. 1. Department of Neurological and Movement Sciences, University of Verona, Italy. 2. Canadian Center for Activity and Aging, School of Kinesiology, University of Western Ontario, Canada. 3. Department of Neurological and Movement Sciences, University of Verona, Italy; Faculty of Physical Activity and Sport, University of Flores, Argentina. 4. Canadian Center for Activity and Aging, School of Kinesiology, University of Western Ontario, Canada; Faculty of Kinesiology, University of Calgary, Canada. 5. Department of Neurological and Movement Sciences, University of Verona, Italy. Electronic address: silvia.pogliaghi@univr.it.
Abstract
OBJECTIVES: We tested the hypothesis that the respiratory compensation point can be accurately determined in healthy participants during incremental cycling exercise using non-invasive near-infrared spectroscopy-derived measures of deoxygenated hemoglobin (deoxyHb). DESIGN: Validation study. METHODS: 118 healthy men (average age 47 ± 19 yrs, range 20-79 yrs) performed an incremental cycling test to exhaustion. Breath-by-breath pulmonary oxygen uptake (VO2) and other ventilatory and gas exchange variables were measured and used to determine respiratory compensation point. Vastus lateralis deoxyHb was monitored using a frequency domain multi-distance system near-infrared spectroscopy device and deoxyHb data were modeled with a piece-wise double-linear function from which the deoxyHb deflection point (deoxyHbDP) was determined. The absolute (L min(-1)) and relative (% maximal VO2 [VO 2max]) VO2 values associated with the respiratory compensation point and deoxyHbDP were determined for each individual. RESULTS: DeoxyHb increased as a function of exercise intensity up to a point (deoxyHbDP) after which the signal displayed a "near-plateau". The deoxyHbDP corresponded to a VO2 of 2.25 ± 0.69 L min(-1) (74 ± 12% VO 2max) which was not significantly different from the VO2 at respiratory compensation point (2.28 ± 0.70 L min(-1) and 74 ± 10% VO 2max, p < 0.05). Both indices were highly correlated (r(2) = 0.86) and Bland Altman analyses confirmed a non-significant bias for VO2 (-0.024 L min(-1)) concomitant with a small imprecision of 0.26 L min(-1). CONCLUSIONS: During incremental cycling exercise, the VO2 associated with the onset of a plateau in near-infrared spectroscopy-derived deoxyHb occurs in coincidence with the VO2 at respiratory compensation point suggesting that respiratory compensation point can be accurately estimated, non-invasively, using near-infrared spectroscopy-derived deoxyHb in alternative to the use of ventilatory-based techniques.
OBJECTIVES: We tested the hypothesis that the respiratory compensation point can be accurately determined in healthy participants during incremental cycling exercise using non-invasive near-infrared spectroscopy-derived measures of deoxygenated hemoglobin (deoxyHb). DESIGN: Validation study. METHODS: 118 healthy men (average age 47 ± 19 yrs, range 20-79 yrs) performed an incremental cycling test to exhaustion. Breath-by-breath pulmonary oxygen uptake (VO2) and other ventilatory and gas exchange variables were measured and used to determine respiratory compensation point. Vastus lateralis deoxyHb was monitored using a frequency domain multi-distance system near-infrared spectroscopy device and deoxyHb data were modeled with a piece-wise double-linear function from which the deoxyHb deflection point (deoxyHbDP) was determined. The absolute (L min(-1)) and relative (% maximal VO2 [VO 2max]) VO2 values associated with the respiratory compensation point and deoxyHbDP were determined for each individual. RESULTS: DeoxyHb increased as a function of exercise intensity up to a point (deoxyHbDP) after which the signal displayed a "near-plateau". The deoxyHbDP corresponded to a VO2 of 2.25 ± 0.69 L min(-1) (74 ± 12% VO 2max) which was not significantly different from the VO2 at respiratory compensation point (2.28 ± 0.70 L min(-1) and 74 ± 10% VO 2max, p < 0.05). Both indices were highly correlated (r(2) = 0.86) and Bland Altman analyses confirmed a non-significant bias for VO2 (-0.024 L min(-1)) concomitant with a small imprecision of 0.26 L min(-1). CONCLUSIONS: During incremental cycling exercise, the VO2 associated with the onset of a plateau in near-infrared spectroscopy-derived deoxyHb occurs in coincidence with the VO2 at respiratory compensation point suggesting that respiratory compensation point can be accurately estimated, non-invasively, using near-infrared spectroscopy-derived deoxyHb in alternative to the use of ventilatory-based techniques.
Authors: Stephan van der Zwaard; Richard T Jaspers; Ilse J Blokland; Chantal Achterberg; Jurrian M Visser; Anne R den Uil; Mathijs J Hofmijster; Koen Levels; Dionne A Noordhof; Arnold de Haan; Jos J de Koning; Willem J van der Laarse; Cornelis J de Ruiter Journal: PLoS One Date: 2016-09-15 Impact factor: 3.240