Literature DB >> 2514229

An enzyme-linked immunoassay for the measurement of rat alpha 1-acid glycoprotein synthesized by cultured hepatocytes.

D Biou1, M Daveau, O Rigal, M Hiron, D Porquet, J P Lebreton.   

Abstract

We have developed a sandwich ELISA to quantify rat alpha 1-acid glycoprotein (AGP). The assay correlated well with RID and the minimum detectable concentration was 1 microgram/l. The assay permits high sensitivity determinations of the rate of synthesis of AGP in vitro. The maximum mean rates observed were 1500 and 1800 ng/24 h/10(6) cells for hepatocytes cultured alone and co-cultured hepatocytes respectively and 39 ng/h/10(6) cells for isolated hepatocytes.

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Year:  1989        PMID: 2514229     DOI: 10.1016/0022-1759(89)90070-7

Source DB:  PubMed          Journal:  J Immunol Methods        ISSN: 0022-1759            Impact factor:   2.303


  2 in total

1.  Effects of insulin, dexamethasone and cytokines on alpha 1-acid glycoprotein gene expression in primary cultures of normal rat hepatocytes.

Authors:  B Barraud; S Balavoine; G Feldmann; B Lardeux
Journal:  Inflammation       Date:  1996-04       Impact factor: 4.092

2.  Evaluation of the hepatotoxicological effects of a drug in an in vivo/in vitro model.

Authors:  D Porquet; M Appel; T Fournier; O Bertaux; D Biou; J Féger
Journal:  Experientia       Date:  1992-03-15
  2 in total

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