Literature DB >> 1372265

Evaluation of the hepatotoxicological effects of a drug in an in vivo/in vitro model.

D Porquet1, M Appel, T Fournier, O Bertaux, D Biou, J Féger.   

Abstract

Both in vivo and in vitro models have certain disadvantages for the study of the chronic hepatotoxicity of drugs. The aim of this work was to evaluate a new approach based on an in vivo/in vitro model. After chronic in vivo treatment of rats with Vincamine and Vindeburnol (an eburnamenine derivative which exhibits hepatotoxic properties in man) liver cells were isolated, and functional and metabolic disorders (metabolic utilization of fructose and protein biosynthesis) were studied to determine injury. The results showed no modification of blood parameters, but a direct relationship between the dose of Vindeburnol administered in vivo and the metabolic disorders observed in vitro, evidencing the high sensitivity and reliability of this model.

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Year:  1992        PMID： 1372265     DOI： 10.1007/bf01930469

Source DB:  PubMed          Journal:  Experientia        ISSN： 0014-4754


  13 in total

1.  The rat serum albumin gene: analysis of cloned sequences.

Authors:  T D Sargent; J R Wu; J M Sala-Trepat; R B Wallace; A A Reyes; J Bonner
Journal:  Proc Natl Acad Sci U S A       Date:  1979-07       Impact factor: 11.205

2.  An enzyme-linked immunoassay for the measurement of rat alpha 1-acid glycoprotein synthesized by cultured hepatocytes.

Authors:  D Biou; M Daveau; O Rigal; M Hiron; D Porquet; J P Lebreton
Journal:  J Immunol Methods       Date:  1989-12-20       Impact factor: 2.303

3.  Immunochemical quantitation of antigens by single radial immunodiffusion.

Authors:  G Mancini; A O Carbonara; J F Heremans
Journal:  Immunochemistry       Date:  1965-09

4.  Rat alpha 1-acid glycoprotein mRNA. Cloning of double-stranded cDNA and kinetics of induction of mRNA levels following acute inflammation.

Authors:  G A Ricca; R W Hamilton; J W McLean; A Conn; J E Kalinyak; J M Taylor
Journal:  J Biol Chem       Date:  1981-10-25       Impact factor: 5.157

5.  [Isolated hepatocytes fixed on collagen, an accurate model for the the secretion of proteins in a minimal medium. Response to inflammation].

Authors:  J Davy; M Appel; D Biou; J Feger; J Agneray
Journal:  Biol Cell       Date:  1983       Impact factor: 4.458

6.  Effect of chlorpromazine on isolated rat hepatocytes.

Authors:  S C Tsao; T Iga; Y Sugiyama; M Hanano
Journal:  Biochem Pharmacol       Date:  1982-02-15       Impact factor: 5.858

7.  Isolation and maintenance of monolayer hepatocytes from the livers of toxin-treated rats.

Authors:  J R MacDonald; K J Thayer; E A Smuckler
Journal:  Exp Mol Pathol       Date:  1987-02       Impact factor: 3.362

8.  Lipogenesis from U14C lactate in obese Zucker rat hepatocytes. Effect of albumin-bound oleate.

Authors:  D Porquet; N Serbource-Goguel; G Durand; J Maccario; J Féger; J Agneray
Journal:  Life Sci       Date:  1984-09-10       Impact factor: 5.037

9.  [Fructose utilization by hepatocytes isolated from fed obese fa/fa Zucker rats. Metabolic fate of fructose in the absence of the modulator].

Authors:  N Serbource-Goguel; D Porquet; J Feger; G Durand; M Appel; J Agneray
Journal:  Ann Nutr Metab       Date:  1985       Impact factor: 3.374

10.  High-yield preparation of isolated rat liver parenchymal cells: a biochemical and fine structural study.

Authors:  M N Berry; D S Friend
Journal:  J Cell Biol       Date:  1969-12       Impact factor: 10.539
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  1 in total

1.  Vincamine Modulates the Effect of Pantoprazole in Renal Ischemia/Reperfusion Injury by Attenuating MAPK and Apoptosis Signaling Pathways.

Authors:  Michael A Fawzy; Sherif A Maher; Mahmoud A El-Rehany; Nermeen N Welson; Nisreen K A Albezrah; Gaber El-Saber Batiha; Moustafa Fathy
Journal:  Molecules       Date:  2022-02-18       Impact factor: 4.411
  1 in total

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