Chinthamony Arul Raj1, Paramasivam Ragavendran1, Dominic Sophia1, Thangarajan Starlin2, Muthian Ahalliya Rathi3, Velliyur Kanniappan Gopalakrishnan4,5,6. 1. Department of Biochemistry, Karpagam University, Coimbatore, Tamil Nadu, India, 641021. 2. Department of Bioinformatics, Karpagam University, Coimbatore, Tamil Nadu, India, 641021. 3. Department of Biochemistry, Sree Narayana Guru College of Arts and Science, KG. Chavadi, Coimbator, Tamil Nadu, 641021, India. 4. Department of Biochemistry, Karpagam University, Coimbatore, Tamil Nadu, India, 641021. vkgopalakrishnan@gmail.com. 5. Department of Bioinformatics, Karpagam University, Coimbatore, Tamil Nadu, India, 641021. vkgopalakrishnan@gmail.com. 6. Department of Biochemistry, Sree Narayana Guru College of Arts and Science, KG. Chavadi, Coimbator, Tamil Nadu, 641021, India. vkgopalakrishnan@gmail.com.
Abstract
OBJECTIVE: To evaluate the enzymatic and non-enzymatic antioxidants of leaf extract from Alpinia purpurata. METHODS: One gram of fresh leaf of Alpinia purpurata was grinded in 2 mL of 50% ethanol and centrifuged at 10,000×g at 4°C for 10 min. The supernatant obtained was used within 4 h for various enzymatic antioxidants assays like superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione S-transferase (GST), ascorbate oxidase, peroxidase, polyphenol oxidase (PPO) and non-enzymatic antioxidants such as vitamin C, total reduced glutathione (TRG) and lipid peroxidation (LPO). RESULTS: The leaf extract of Alpinia purpurata possess antioxidants like vitamin C 472.92±6.80 μg/mg protein, GST 372.11±5.70 μmol of 1-chloro 2,4 dinitrobenzene (CDNB)-reduced glutathione (GSH) conjugate formed/min/mg protein, GPx 281.69±6.43 μg of glutathione oxidized/min/mg protein, peroxidases 173.12±9.40 μmol/g tissue, TRG 75.27±3.55 μg/mg protein, SOD 58.03±2.11 U/mg protein, CAT 46.70±2.35 μmol of H2O2 consumed/min/mg protein in high amount whereas ascorbate oxidase 17.41±2.46 U/g tissue, LPO 2.71±0.14 nmol/L of malondialdehyde formed/min/mg protein and PPO 1.14±0.11 μmol/g tissue in moderate amount. CONCLUSION: Alpinia purpurata has the potential to scavenge the free radicals and protect against oxidative stress causing diseases. In future, Alpinia purpurata may serve as a good pharmacotherapeutic agent.
OBJECTIVE: To evaluate the enzymatic and non-enzymatic antioxidants of leaf extract from Alpinia purpurata. METHODS: One gram of fresh leaf of Alpinia purpurata was grinded in 2 mL of 50% ethanol and centrifuged at 10,000×g at 4°C for 10 min. The supernatant obtained was used within 4 h for various enzymatic antioxidants assays like superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione S-transferase (GST), ascorbate oxidase, peroxidase, polyphenol oxidase (PPO) and non-enzymatic antioxidants such as vitamin C, total reduced glutathione (TRG) and lipid peroxidation (LPO). RESULTS: The leaf extract of Alpinia purpurata possess antioxidants like vitamin C 472.92±6.80 μg/mg protein, GST 372.11±5.70 μmol of 1-chloro 2,4 dinitrobenzene (CDNB)-reduced glutathione (GSH) conjugate formed/min/mg protein, GPx 281.69±6.43 μg of glutathione oxidized/min/mg protein, peroxidases 173.12±9.40 μmol/g tissue, TRG 75.27±3.55 μg/mg protein, SOD 58.03±2.11 U/mg protein, CAT 46.70±2.35 μmol of H2O2 consumed/min/mg protein in high amount whereas ascorbate oxidase 17.41±2.46 U/g tissue, LPO 2.71±0.14 nmol/L of malondialdehyde formed/min/mg protein and PPO 1.14±0.11 μmol/g tissue in moderate amount. CONCLUSION:Alpinia purpurata has the potential to scavenge the free radicals and protect against oxidative stress causing diseases. In future, Alpinia purpurata may serve as a good pharmacotherapeutic agent.
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