Literature DB >> 25139047

Structure of the full-length insecticidal protein Cry1Ac reveals intriguing details of toxin packaging into in vivo formed crystals.

Artem G Evdokimov1, Farhad Moshiri, Eric J Sturman, Timothy J Rydel, Meiying Zheng, Jeffrey W Seale, Sonya Franklin.   

Abstract

For almost half a century, the structure of the full-length Bacillus thuringiensis (Bt) insecticidal protein Cry1Ac has eluded researchers, since Bt-derived crystals were first characterized in 1965. Having finally solved this structure we report intriguing details of the lattice-based interactions between the toxic core of the protein and the protoxin domains. The structure provides concrete evidence for the function of the protoxin as an enhancer of native crystal packing and stability.
© 2014 The Protein Society.

Entities:  

Keywords:  Bacillus thuringiensis; Cry1A; insecticidal toxin; insecticidal toxin mode of action; natural crystal packing; structure of full length toxin

Mesh：

Substances：

Year:  2014        PMID： 25139047      PMCID： PMC4241100          DOI： 10.1002/pro.2536

Source DB:  PubMed          Journal:  Protein Sci        ISSN： 0961-8368            Impact factor:   6.725


  27 in total

Review 1.  How Bacillus thuringiensis has evolved specific toxins to colonize the insect world.

Authors:  R A de Maagd; A Bravo; N Crickmore
Journal:  Trends Genet       Date:  2001-04       Impact factor: 11.639

2.  Structures of two repeats of spectrin suggest models of flexibility.

Authors:  V L Grum; D Li; R I MacDonald; A Mondragón
Journal:  Cell       Date:  1999-08-20       Impact factor: 41.582

3.  Refinement of macromolecular structures by the maximum-likelihood method.

Authors:  G N Murshudov; A A Vagin; E J Dodson
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  1997-05-01

4.  Coot: model-building tools for molecular graphics.

Authors:  Paul Emsley; Kevin Cowtan
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2004-11-26

Review 5.  Role of receptor interaction in the mode of action of insecticidal Cry and Cyt toxins produced by Bacillus thuringiensis.

Authors:  I Gómez; L Pardo-López; C Muñoz-Garay; L E Fernandez; C Pérez; J Sánchez; M Soberón; A Bravo
Journal:  Peptides       Date:  2006-12-04       Impact factor: 3.750

6.  Polydispersity of Bacillus thuringiensis Cry1 toxins in solution and its effect on receptor binding kinetics.

Authors:  Luke Masson; Alberto Mazza; Sreedhara Sangadala; Michael J Adang; Roland Brousseau
Journal:  Biochim Biophys Acta       Date:  2002-02-11

Review 7.  Mode of action of mosquitocidal Bacillus thuringiensis toxins.

Authors:  Mario Soberón; Luisa E Fernández; Claudia Pérez; Sarjeet S Gill; Alejandra Bravo
Journal:  Toxicon       Date:  2006-11-21       Impact factor: 3.033

8.  Development and commercial use of Bollgard cotton in the USA--early promises versus today's reality.

Authors:  F J Perlak; M Oppenhuizen; K Gustafson; R Voth; S Sivasupramaniam; D Heering; B Carey; R A Ihrig; J K Roberts
Journal:  Plant J       Date:  2001-09       Impact factor: 6.417

9.  Protein production by auto-induction in high density shaking cultures.

Authors:  F William Studier
Journal:  Protein Expr Purif       Date:  2005-05       Impact factor: 1.650

10.  Insect resistant cotton plants.

Authors:  F J Perlak; R W Deaton; T A Armstrong; R L Fuchs; S R Sims; J T Greenplate; D A Fischhoff
Journal:  Biotechnology (N Y)       Date:  1990-10
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  16 in total

Review 1.  In vivo protein crystallization in combination with highly brilliant radiation sources offers novel opportunities for the structural analysis of post-translationally modified eukaryotic proteins.

Authors:  Michael Duszenko; Lars Redecke; Celestin Nzanzu Mudogo; Benjamin Philip Sommer; Stefan Mogk; Dominik Oberthuer; Christian Betzel
Journal:  Acta Crystallogr F Struct Biol Commun       Date:  2015-07-29       Impact factor: 1.056

2.  Use of Redundant Exclusion PCR To Identify a Novel Bacillus thuringiensis Cry8 Toxin Gene from Pooled Genomic DNA.

Authors:  Fengjiao Zhang; Changlong Shu; Neil Crickmore; Yanqiu Li; Fuping Song; Chunqin Liu; Zhibao Chen; Jie Zhang
Journal:  Appl Environ Microbiol       Date:  2016-06-13       Impact factor: 4.792

3.  Cry6Aa1, a Bacillus thuringiensis nematocidal and insecticidal toxin, forms pores in planar lipid bilayers at extremely low concentrations and without the need of proteolytic processing.

Authors:  Eva Fortea; Vincent Lemieux; Léna Potvin; Vimbai Chikwana; Samantha Griffin; Timothy Hey; David McCaskill; Kenneth Narva; Sek Yee Tan; Xiaoping Xu; Vincent Vachon; Jean-Louis Schwartz
Journal:  J Biol Chem       Date:  2017-06-16       Impact factor: 5.157

4.  Insecticidal Specificity of Cry1Ah to Helicoverpa armigera Is Determined by Binding of APN1 via Domain II Loops 2 and 3.

Authors:  Zishan Zhou; Yuxiao Liu; Gemei Liang; Yongping Huang; Alejandra Bravo; Mario Soberón; Fuping Song; Xueping Zhou; Jie Zhang
Journal:  Appl Environ Microbiol       Date:  2017-02-01       Impact factor: 4.792

5.  The C-terminal protoxin region of Bacillus thuringiensis Cry1Ab toxin has a functional role in binding to GPI-anchored receptors in the insect midgut.

Authors:  Arlen Peña-Cardeña; Ricardo Grande; Jorge Sánchez; Bruce E Tabashnik; Alejandra Bravo; Mario Soberón; Isabel Gómez
Journal:  J Biol Chem       Date:  2018-11-01       Impact factor: 5.157

Review 6.  The Essential and Enigmatic Role of ABC Transporters in Bt Resistance of Noctuids and Other Insect Pests of Agriculture.

Authors:  David G Heckel
Journal:  Insects       Date:  2021-04-28       Impact factor: 2.769

Review 7.  Bacillus thuringiensis toxins: an overview of their biocidal activity.

Authors:  Leopoldo Palma; Delia Muñoz; Colin Berry; Jesús Murillo; Primitivo Caballero
Journal:  Toxins (Basel)       Date:  2014-12-11       Impact factor: 4.546

Review 8.  Which Is Stronger? A Continuing Battle Between Cry Toxins and Insects.

Authors:  Lu Liu; Zhou Li; Xing Luo; Xia Zhang; Shan-Ho Chou; Jieping Wang; Jin He
Journal:  Front Microbiol       Date:  2021-06-01       Impact factor: 5.640

9.  Dual mode of action of Bt proteins: protoxin efficacy against resistant insects.

Authors:  Bruce E Tabashnik; Min Zhang; Jeffrey A Fabrick; Yidong Wu; Meijing Gao; Fangneng Huang; Jizhen Wei; Jie Zhang; Alexander Yelich; Gopalan C Unnithan; Alejandra Bravo; Mario Soberón; Yves Carrière; Xianchun Li
Journal:  Sci Rep       Date:  2015-10-12       Impact factor: 4.379

10.  The pesticidal Cry6Aa toxin from Bacillus thuringiensis is structurally similar to HlyE-family alpha pore-forming toxins.

Authors:  Alexey Dementiev; Jason Board; Anand Sitaram; Timothy Hey; Matthew S Kelker; Xiaoping Xu; Yan Hu; Cristian Vidal-Quist; Vimbai Chikwana; Samantha Griffin; David McCaskill; Nick X Wang; Shao-Ching Hung; Michael K Chan; Marianne M Lee; Jessica Hughes; Alice Wegener; Raffi V Aroian; Kenneth E Narva; Colin Berry
Journal:  BMC Biol       Date:  2016-08-30       Impact factor: 7.431
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