Himani Bhardwaj Pandya1, Jagdish Shantilal Patel2, Harihar Hardas Agravat3, Navneet Kumar Ramdayal Singh4. 1. Assistant Professor, Department of Microbiology, Shree P. M. Patel College of Paramedical Science and Technology , Anand, Gujarat, India . 2. Head of the Department, Department of Biochemistry, P.D. Patel Institute of Applied Sciences, Charotar University of Science and Technology (CHARUSAT) , Changa, Gujarat, India . 3. Dean, C. U, Shah Medical College , Surendranagar, Gujarat, India . 4. Assistant Professor (Medical Microbiology), Department of Microbiology, Shree P. M. Patel College of Paramedical Science and Technology , Anand, Gujarat, India .
Abstract
CONTEXT: Validation of an accurate and less cumbersome noninvasive method to detect current Helicobacter pylori infection is a requisite for any laboratory. OBJECTIVES: The purpose of this study is to corroborate the usefulness of two commercially available kits NovaTec ELISA-A and ELISA-G, in the detection of ongoing H.pylori infection. MATERIALS AND METHODS: Two hundred and twenty eight consecutive serum samples of symptomatic patients who attended the endoscopy unit of "Deep" surgical hospital, Anand, which were collected during the period from 27th February 2008 to 31st august 2011, were studied. The sera were processed and tested for the detection of the H.pylori IgG and IgA antibody by using a solid phase; capture micro well ELISA, procured from Nova Tec immunodiagnostica GmbH Germany. RESULTS: IgG ELISA showed 100% sensitivity and Negative predictive value (NPV), while IgA ELISA was better in terms of specificity (61.4%) and accuracy (63%) as compared to IgG ELISA. We found 7% (16/228) of IgA positive cases with IgG negative response. IgG response was more common in reflux esophagitis patients (OR 1.451, 95%CI-0.850-2.477) and then in gastritis (OR 0.962, 95%CI-0.570-1.621) and duodenitis(OR-0.806, 95%CI-0.112-5.827), while IgA positive response was more common in duodenitis patients (OR-1.383, 95%CI-0.191-9.995) and reflux esophagitis patients (OR 1.289, 95% CI-0.756-2.197) and least in duodenal ulcer patients (OR 0.670, 95%CI-0.222-2.029). CONCLUSION: IgG update is reliable and accurate test and can be expedient as a screening test and thus serve as an alternative to endoscopy. For the purpose of excluding infection with H.pylori, the performance of IgG is moderate (low specificity) but can be improved by conjunctional IgA testing which will offer some additional diagnostic value.
CONTEXT: Validation of an accurate and less cumbersome noninvasive method to detect current Helicobacter pyloriinfection is a requisite for any laboratory. OBJECTIVES: The purpose of this study is to corroborate the usefulness of two commercially available kits NovaTec ELISA-A and ELISA-G, in the detection of ongoing H.pylori infection. MATERIALS AND METHODS: Two hundred and twenty eight consecutive serum samples of symptomatic patients who attended the endoscopy unit of "Deep" surgical hospital, Anand, which were collected during the period from 27th February 2008 to 31st august 2011, were studied. The sera were processed and tested for the detection of the H.pylori IgG and IgA antibody by using a solid phase; capture micro well ELISA, procured from Nova Tec immunodiagnostica GmbH Germany. RESULTS: IgG ELISA showed 100% sensitivity and Negative predictive value (NPV), while IgA ELISA was better in terms of specificity (61.4%) and accuracy (63%) as compared to IgG ELISA. We found 7% (16/228) of IgA positive cases with IgG negative response. IgG response was more common in reflux esophagitispatients (OR 1.451, 95%CI-0.850-2.477) and then in gastritis (OR 0.962, 95%CI-0.570-1.621) and duodenitis(OR-0.806, 95%CI-0.112-5.827), while IgA positive response was more common in duodenitispatients (OR-1.383, 95%CI-0.191-9.995) and reflux esophagitispatients (OR 1.289, 95% CI-0.756-2.197) and least in duodenal ulcerpatients (OR 0.670, 95%CI-0.222-2.029). CONCLUSION: IgG update is reliable and accurate test and can be expedient as a screening test and thus serve as an alternative to endoscopy. For the purpose of excluding infection with H.pylori, the performance of IgG is moderate (low specificity) but can be improved by conjunctional IgA testing which will offer some additional diagnostic value.
Entities:
Keywords:
Enzyme linked immunosorbant assay (ELISA); Helicobacter pylori; Immunoglobulin A (IgA); Immunoglobulin G (IgG)
Authors: M Mohammadi; Y Talebkhan; G Khalili; F Mahboudi; S Massarrat; L Zamaninia; A Oghalaei Journal: Indian J Med Microbiol Date: 2008 Apr-Jun Impact factor: 0.985
Authors: Raul V Destura; Eternity D Labio; Leah J Barrett; Cirle S Alcantara; Venancio I Gloria; Ma Lourdes O Daez; Richard L Guerrant Journal: Ann Clin Microbiol Antimicrob Date: 2004-11-16 Impact factor: 3.944