Literature DB >> 2511803

Rapid nucleic acid assay for detection of bacteria with tetM-mediated tetracycline resistance.

R Sanchez-Pescador1, J A Running, M M Stempien, M S Urdea.   

Abstract

A novel nucleic acid assay has been developed to screen bacterial populations for the presence of the tetM structural gene. The method involves the specific hybridization of several synthetic oligonucleotides to the gene in a crude bacterial lysate solution. As few as 1.5 x 10(4) CFU can be detected with the assay.

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Year:  1989        PMID： 2511803      PMCID： PMC172761          DOI： 10.1128/AAC.33.10.1813

Source DB:  PubMed          Journal:  Antimicrob Agents Chemother        ISSN： 0066-4804            Impact factor:   5.191


  15 in total

1.  High-level tetracycline resistance in Neisseria gonorrhoeae is result of acquisition of streptococcal tetM determinant.

Authors:  S A Morse; S R Johnson; J W Biddle; M C Roberts
Journal:  Antimicrob Agents Chemother       Date:  1986-11       Impact factor: 5.191

2.  The nucleotide sequence of the tetracycline resistance determinant tetM from Ureaplasma urealyticum.

Authors:  R Sanchez-Pescador; J T Brown; M Roberts; M S Urdea
Journal:  Nucleic Acids Res       Date:  1988-02-11       Impact factor: 16.971

Review 3.  Use of nonradioactive DNA probes for the detection of infectious bacteria.

Authors:  M Pollice; H L Yang
Journal:  Clin Lab Med       Date:  1985-09       Impact factor: 1.935

4.  Nucleotide sequence of the tetM tetracycline resistance determinant of the streptococcal conjugative shuttle transposon Tn1545.

Authors:  P Martin; P Trieu-Cuot; P Courvalin
Journal:  Nucleic Acids Res       Date:  1986-09-11       Impact factor: 16.971

5.  Dissemination of the tetM tetracycline resistance determinant to Ureaplasma urealyticum.

Authors:  M C Roberts; G E Kenny
Journal:  Antimicrob Agents Chemother       Date:  1986-02       Impact factor: 5.191

6.  Penicillinase-producing Gonococci in Liverpool.

Authors:  A Percival; J Rowlands; J E Corkill; C D Alergant; O P Arya; E Rees; E H Annels
Journal:  Lancet       Date:  1976-12-25       Impact factor: 79.321

7.  Tetracycline resistance and tetM in pathogenic urogenital bacteria.

Authors:  M C Roberts; S L Hillier; J Hale; K K Holmes; G E Kenny
Journal:  Antimicrob Agents Chemother       Date:  1986-11       Impact factor: 5.191

8.  DNA probes for identification of tetracycline resistance genes in Campylobacter species isolated from swine and cattle.

Authors:  L K Ng; M E Stiles; D E Taylor
Journal:  Antimicrob Agents Chemother       Date:  1987-11       Impact factor: 5.191

9.  NEISSERIA GONORRHOEAE. I. VIRULENCE GENETICALLY LINKED TO CLONAL VARIATION.

Authors:  D S KELLOGG; W L PEACOCK; W E DEACON; L BROWN; D I PIRKLE
Journal:  J Bacteriol       Date:  1963-06       Impact factor: 3.490

10.  Tetracycline-resistant Mycoplasma hominis strains contain streptococcal tetM sequences.

Authors:  M C Roberts; L A Koutsky; K K Holmes; D J LeBlanc; G E Kenny
Journal:  Antimicrob Agents Chemother       Date:  1985-07       Impact factor: 5.191
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